固定化青霉菌吸附活性艳蓝KN-R的脱色研究  

Study on decolorization of reactive birlliant blue KN-R by the adsorption of immobilized Penicillinm jensenii

作　　者：董新姣[1] 周国玲[1] 

机构地区：[1]温州大学生命与环境科学学院

出　　处：《中国环境科学》2005年第4期494-498,共5页China Environmental Science

基　　金：浙江省自然科学基金资助项目(Y504233);浙江省教育厅科研基金资助项目(20040611)

摘　　要：利用植物载体玉米芯对青霉菌X5进行了固定化研究.通过正交试验确定载体固定化真菌的最优条件,考察了pH值、温度、盐浓度、葡萄糖浓度对菌株脱色的影响.结果表明,菌量为8g/L,载体大小为1/3×1cm2×π×1cm,载体个数为15个,摇床转速为100r/min条件下玉米芯能够有效地固定菌体,固定化菌对染料脱色的最佳温度为30℃,pH值为3.0,葡萄糖浓度为10g/L,盐度对脱色有一定的影响,在最优条件下,固定化菌对活性艳蓝KN-R脱色率达到95%以上.固定化菌对活性艳蓝脱色符合二级动力学方程,生物吸附过程较好地符合Freundlich吸附模型,固定化菌体重复利用5次后,脱色率仍达73.51%.The immobilization studies on the penicillium jensenii was carried out utilizing plant-carrier, corn bar. The optimal condition for the strain immobilization of the medium was determined through orthogonal test. The influence of pH value, temperature, salinity and glucose concentration on the strain decolorizafion was investigated. The corn bar could immobilize effectively the strain under the conditions of the strain weight of 8g/L, the carrier size of 1/3×1cm^2×π×1cm, the carrier number of 15, the shaker speed of 100r/min; the optimum temperature and pH value were 30℃ and 3.0, the concentration of glucose was 10mg/L for the dye decolorization of immobilized strain; the concentration of salinity had definite influence on decolorization; the decolorization rate of the immobilized strain on reactive brilliant blue KN-R reached above 95% under the optimum condition, conforming to second-order dynamic equation. The bioadsorption process conformed relatively good to Freundlich adsorption model. The decolorization rate of the immobilized strain after reused for five times could still reach 73.51%.

关 键 词：染料脱色 青霉菌 固定化 植物载体 

分 类 号：X172[环境科学与工程—环境科学]