柿疯病研究Ⅱ.病原  

STUDIES OF PERSIMMON PROLIFERATION DISEASE II.ETIOLOGY

作　　者：王祈楷[1] 刘宏迪[1] 冯鲁昕 俎显诗 张良皖 张金岐[2] 刘秋芬[2] 

机构地区：[1]中国科学院微生物研究所 [2]河北省农林科学院昌黎果树研究所

出　　处：《植物病理学报》1989年第1期7-9,共3页Acta Phytopathologica Sinica

摘　　要：从柿疯病株的1—2年生枝条或根的输导组织中,抽取组织液并离心富集制备样品,经磷钨酸钠负染,和Epon812树脂常规包埋超薄切片,在电镜下检查很多多型性原核细胞,大小不一,长580—1600nm,宽510—1060nm,胞壁呈山脊状波纹,厚约25nm,无鞭毛而周身分布微小疣突,按其形状大小和亚显微结构特征确定为难养细菌(Fostidious bacteria)而不同于一般植物病原细菌,健株制备的样品中未检查到任何微生物。这个结果与前文报道青霉素可抑制和缓解柿疯病症状表现的结果是一致的[1],检查到的这种难养细菌认为是柿疯病的病原物。前已证明,柿疯病可以嫁接传病,并对该病的症状作了系统的观察和描述[1],本文报道柿疯病病原的研究结果。The preparations were separated and concentrated with differential centrifuga-tion from vascular tissue sap which had been pumpted from twig or root segments of proliferation diseased and healthy persimmon.They were obseved by me- ans of negative staining and ultrathin section with electron microscopy.Nu me-rous pleomorphic procaryotic cells were found exclusively in the preparations m-ade from diseased plants.They are of varying sizes,usually 580—1600 nm in length and 510—1060 nm in width.The cell wall looks ripply externally,about 25 nm in thickness.No flagella but several prosthecae occur on the surface of the cells.The pleomorphic procaryote in question with all those cytological characteristics is distinct obviously from ordinary bacteria and it was identifi-ed as a sort of fastidious b(?)cteria.There is no microbe whatsoever to be found within the samples made from PPD-free pesimmon.This result is consistent with that of formal researches which elusidated the symptoms of PPD could be depressed by treatment with enicillin. Therefore , the fastidious bacterium seems to be the pathogen of PPD.

关 键 词：柿疯病研究 柿疯病病原 

分 类 号：S436.45[农业科学—农业昆虫与害虫防治]