用改良的寡核苷酸诱导的定位突变技术构建组织型纤溶酶原激活剂cDNA突变体  

Studies on construction of tissue-type plasminogen activator mutants by modified oligonucleotide-mediated site-directed mutagenesis techniques

作　　者：刘士辉[1] 黄培堂[1] 黄翠芬[1] 

机构地区：[1]北京生物工程研究所,北京100850

出　　处：《军事医学科学院院刊》1995年第1期13-19,共7页Bulletin of the Academy of Military Medical Sciences

摘　　要：为了得到性能改善的组织型纤溶酶原激活剂（ｔ－ＰＡ）突变体，运用Ｐｒｏｍｅｇａ公司研制的改良的寡核苷酸诱导的定位突变技术，成功地构建了 ｔ－ＰＡ ｃＤＮＡ定位点突变、缺失突变及置换突变等多种突变体。ＤＮＡ序列分析表明突变频率达７５％以上。In order to improve the current tissue-type plasminogen activator(t-PA) preparations, the authors successfully constructed several site-directed point, deletion and replacement mutants of t-PA by the method of oligonucleotide-mediated site-directed mutagenesis modified by Promega Corporation. The DNA sequence analyses demonstrated that the rates of mutagenesis were more than 75 persent.

关 键 词：组织型 纤溶酶原激活剂 寡核苷酸 定位突变 

分 类 号：R392-33[医药卫生—免疫学]