癌性胸腹水TIL体外抗肿瘤作用研究  

In Vitro Antitumor Activitv of TIL from Human Carcinous Exudate of Cancer Patients

作　　者：姜平[1] 王秦秦[1] 陈新明[1] 李继梅[1] 郝萍[1] 

机构地区：[1]昆明医学院第一附属医院肿瘤研究所

出　　处：《昆明医学院学报》1995年第4期21-22,共2页Journal of Kunming Medical College

基　　金：云南省教委青年教师基金

摘　　要：采用不连续密度梯度分离法分别从３例肺癌胸水和１例胰头癌腹水中分离、诱导培养出肿瘤浸润淋巴细胞（ＴＩＬ），并用乳酸脱氢酶（ＬＤＨ）释放法分析了白细胞介素Ⅱ培养前后ＴＩＬ的体外抗肿瘤作用．结果表明：新鲜分离的ＴＩＬ对自体肿瘤细胞，Ｋ（５６２）细胞及Ｒａｊｉ细胞杀伤力很低，经ＩＬ－２培养后对上述肿瘤细胞的杀伤能力增强，并随培养时间增强，培养到第１５～２０ｄ杀伤力较强，以后逐渐减弱．ＴＩＬ在体外可生长４０余天．ＴＩＬ对自体肿瘤的杀伤活性明显高于对异体肿瘤的杀伤活性（Ｐ＜０．０５）．对Ｒａｊｉ细胞的杀伤率高于对Ｋ（５６２）细胞的杀伤率．研究认为新鲜分离的ＴＩＬ可能处于一种免疫抑制状态，白细胞介素Ⅱ培养可能解除了这种抑制，而提高了抗瘤活性．这种活性以ＬＡＫ样作用为主．同时，ＩＬ－２培养提高了ＴＩＬ对自身肿瘤的特异细胞毒作用．Tumor-infiltrating lymphocytes（TIL）was separated respectively from carcinous exudate in 3 patients with lung cancer and from ascites in one patients with pancreas cancer by discontininous density gradient centrifugation．The cytotoxity of TIL which cultured with IL-2（interleukin-2) were compared with the fresh TIL just from carcinous exudate．The results showed that TILs culture with IL-2 have higher antltumor activity in vitro than fresh TILs．The antitumor activity of TILs was at highest level between l5－20 days after culture in IL-2 and then declined．The cytotoxity to autotoxity to autologous tumor cells was higher than to K562 and Raji target cells（P＜0．05）． Our results will be beneficial to clinic use of TIL．

关 键 词：肿瘤 胸水 腹水 淋巴细胞 白细胞介素 

分 类 号：R730.3[医药卫生—肿瘤]