尿葡萄糖二酸测定及对药物性肝炎鉴别诊断的评价  

Urine glucaric acid determination and differential diagnosis in hepatitis patient

作　　者：赵允文[1] 杨荣伟[1] 黄卫锋[1] 

机构地区：[1]浙江医科大学附属一院,南通医学院附属医院

出　　处：《临床检验杂志》1995年第5期233-235,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：本文根据酶竞争抑制原理，应用自动生化分析仪测定尿液中葡萄糖二酸（ＵＧＡ），提高了测定效率、精密度和准确性。本法天内ＣＶ为３．１％，回收率９６．５％，线性范围０～１８０μｍｏｌ／Ｌ。４８例健康人ＵＧＡ为１．８～３１．３μｍｏｌ／ｇ．Ｃｒ，非药物性肝炎病人为４．２～５６．８μｍｏｌ／ｇ．Ｃｒ、药物性肝炎病人为１９．６～５５３μｍｏｌ／ｇ．Ｃｒ，以５０μｍｏｌ／ｇ．Ｃｒ作为切割值，其鉴别诊断效率为８７．８％。同时也观察到某些药物对测定有负干扰。On the basis of enzyme competitive inhibiton principle, we designed a simple, high precision method to estimate urine glucaric acid (UGA) with phenolphthalein giucuronic sodium as a substrate.The criteria of analytical performance were: within-run CV 3. 1%, day-to-day CV 4. 2%.average recovery 96. 5% and linear range 0～180 μmol/L. It's confidence limit of 95% was 1.8～31.3(16.6±7.38.x±s) ,19.6～553.3,4.2～56.8 μmol/g. Cr, in health group, drug hepatitis group (DHPD) and nondrug hepatitis group(NDHPD) respectivcly, t test showed: P<0. 001 in DHPD and NDHPD.We use 50 μmol/g. Cr as cut-off value, the positive rate of DHPD and NDHPD was 78. 9%(30/38), 9.2%(10/109) repectively, and the differential diagnosis efficiency was 87. 8%.It had been observed that some drugs could reduce the absorbance of blank. even lower than sample's. We confirmed cipfloxin and some chinese herbs, especially drugs mix-used in kidney transplant patients, may interfere with determination.

关 键 词：葡萄糖二酸 肝炎 尿液 鉴别诊断 

分 类 号：R575.04[医药卫生—消化系统]