桥粒斑蛋白Ⅱ在各种上皮组织中的表达  

Expression of Desmoplakin Ⅱ in Different Epithelia

作　　者：杨举伦[1] 邹红[1] 蔡琳[1] 罗学林[1] 明洪[1] 

机构地区：[1]成都军区昆明总医院,昆明医学院附一院中心实验室

出　　处：《免疫学杂志》1995年第1期40-42,共3页Immunological Journal

基　　金：云南省科委应用基础研究基金

摘　　要：桥粒斑蛋白Ⅱ（ｄｅｓｍｏｐｌａｋｉｎⅡ，ＤＰⅡ）是桥粒的主要蛋白成分之一，起连接细胞内中间丝和桥粒的作用。本文用研制的抗ＤＰⅡ单克隆抗体及免疫组织化学方法，观察ＤＰⅡ在牛、猪、猴、人各种上皮组织和非上皮组织中的表达。结果显示：ＤＰⅡ表达于牛、猪、猴、人的除甲状腺滤泡上皮外的所有被检上皮中，包括复层上皮、单层上皮、假复层上皮和移行上皮。阳性染色点大部分分布于细胞交界面上，少数弥散到细胞浆中。在鳞状上皮细胞的分化过程中，ＤＰⅡ的分布随桥粒的合成和降解而变化。ＤＰⅡ不表达于牛、猪、猴、人的非上皮组织中。上述结果表明，ＤＰⅡ抗原具有种属交叉性；ＤＰⅡ虽然不存在于所有桥粒中，但仍是一种有用的上皮组织标记物。本文还讨论了鳞状上皮中ＤＰⅡ分布与桥粒代谢的关系。Desmoplakin Ⅱ(DP Ⅱ ) which connects intermediate filaments to desmosomes is one of themajor desmosomal proteins.The expression and distributlon of DP Ⅱ in different epithelia and non-epitheliaof bovine,swine,monkey and human were,tudied immunohitstochemically with monoclonal antibody againstDP Ⅱ produced by our laboratory.The results showed that DP Ⅱ expressed in all the tested epithelial tissuesexcept follicular epithelium of throid from bovine,xwine,monkey and human including stratified,transitional,simple and pseudostratified epithelia,Most of positive staining spots located in cell-cell border and some in cy-toplasm. There were no DP Ⅱ expression in all the nonepithelia tested. We also found that during differentia-tion of squamous epithelium DP Ⅱ distribution changed with synthesis and degradation of desmosomes. Ourresults suggext that DP Ⅱ have species-cross antigenicity and is an useful marker for identification of epithe-lial tissue although it is not a constituent of all desmosome.The relation of DP Ⅱ distribution and desmo-some metabolism was discussed in this paper.

关 键 词：桥粒 桥粒斑蛋白 上皮 单克隆抗体 免疫组织化学 

分 类 号：R730.3[医药卫生—肿瘤]