荞麦愈伤组织培养及其分化的研究  

Induction and Differentiation of Callus on Buckwheat

作　　者：侯建华[1] 耿庆汉 

机构地区：[1]内蒙古农牧学院农学系

出　　处：《内蒙古农牧学院学报》1995年第3期21-25,共5页

基　　金：内蒙科委课题

摘　　要：以荞麦的成熟胚，幼胚，下胚轴为外植体，在不同激素浓度配比，不同基本培养基质培养基上的组织培养研究表明：以成熟胚为外植体时，对愈伤组织诱导和生长最适培养基是２．４－Ｄ２．０，ＮＡＡ１．０，６－ＢＡ。０．５的Ｂ＿５培养基；以幼胚为外植体时，对愈伤组织的诱导和生长最适合的培养基是２．４－Ｄ２．０，ＮＡＡ１．０，６－ＢＡ０的ＭＳ培养基；以下胚轴为外植体时，Ｂ＿５和ＭＳ两种培养基均不适合愈伤组织的生长。２．４－０对荞麦愈伤组织根的分化有一定的促进作用。Using mature embryo,young embryo,and hypocotyl of buckwheat asexplant,we studied the induction of callus under different concentrations of promoting substance and different kinds of basal medium.The results were as follows:If embryo of buckwheat was used as explant,the most suitable medium for the induction of callus was the B_5 basal medium supplemented with 2mg/12,4-D,1.0mg/1 NAA,0.5mg/16-BA;if young embry was used as explant,the most suitable medium for the induction of callus was the MS basal medium supplemented with 2mg/12.4-D,1.0mg/l NAA,0mg/1 6-BA;if hypocotyl was used as explant,the B_5 and MS basal medium was not suitable for induction of callus.

关 键 词：愈伤组织 荞麦 外植体 诱导率 

分 类 号：S517.035.3[农业科学—作物学]