大肠杆菌K_（99）─F_（41）基因工程亚单位苗对牛和羊的攻毒保护试验研究  

STUDIES ON PROTECTING CALF AND LAMB AGAINST PATHOGENIC BACTERIUM TEST WITH VACCINATING ESCHERICHIA COLI K99-F41GENG ENGINEERING SUBUNIT VACCINE

作　　者：王玉炯[1] 徐桂珍[1] 刘子[1] 冯书章[1] 许崇波[1] 周吉清[1] 杨鹏[1] 

机构地区：[1]宁夏农学院,解放军农牧大学,宁夏平吉堡奶牛场

出　　处：《宁夏农学院学报》1995年第2期39-42,共4页Journal of Ningxia Agricultural College

摘　　要：用大肠杆菌Ｋ９９─Ｆ４１基因工程亚单位苗以两种剂量（正常免疫剂量和１／５免疫剂量）分别免疫接种怀孕母牛和怀孕母羊，待其所产幼畜（犊牛和羔羊）吃过初乳后，经口灌服致幼畜腹泻的强毒活菌，犊牛５００亿／头，羔羊２５０亿／只。结果，正常免疫剂量组，犊牛腹泻发病率为１９％，死亡率为零；羔羊腹泻发病率为１５％，死亡率为５％。１／５免疫剂量组，犊牛腹泻发病率为４０％，死亡率为１０％；羔羊腹泻发病率为３６．８％，死亡率为１５．８％，而未接种疫苗的对照组，犊牛腹泻发病率为７０％，死亡率为３５％，羔羊腹泻发病率为７０％，死亡率为６５％，经统计学处理，三组之间犊牛和羔羊的发病率和死亡率均呈极显著差异（Ｐ＜０．０１）。The pregnant cows and sheep were vaccinated with Escherichia coli K99 -F41gene engineering subunit vacine in two kinds of doses separately (normal dose and 1/5 normal dose).After feeding primary milk from the dames,the newborn calves and lamds were taken orally pathogenic bacterium that could effect the newborn infants diarrhoes.It was shown that the calves diarrhoea attack rate and death rate were 19. 0%and 0%separately,the lambs diarrhoes were 15% and 5% separately in the normal group;the calves diarrhoes attack rate and death rate 40% and 10% separately,the lambs dirrhoea attack rate and death rate 36 %,8%amd 15. 8 % separately in the 1/5 normal dose group,and in the contrast group without vaccinating;the calves diarrhoes attack rate and death rate 70%and 35 % separately,the lambs dirrhoea attack rate and death rate 70%and 65% separately.There was a significant difference (p<0. 01 )among the attack rate and death rate of the.3 groups by statistical comparision.

关 键 词：大肠杆菌 基因工程 牛 羊 幼畜 腹泻 疫苗 

分 类 号：S855.12[农业科学—临床兽医学] S859.797[农业科学—兽医学]