高敏感薄层平板抗菌肽活性检测  

A Sensitive Thin-layer Activity Assays for Antibacterial Polypeptide

作　　者：夏明明[1] 徐上[1] 李伟[1] 魏万贵[1] 屈贤铭[1] 贾士荣[1] 

机构地区：[1]中国科学院上海生物工程研究中心,上海医科大学眼耳鼻喉医院,中国农业科学院生物技术研究中心

出　　处：《农业生物技术学报》1995年第2期19-24,共6页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划资助项目

摘　　要：本文对迄今通用的平皿琼脂糖孔穴扩散法进行了改进，使检测敏感性提高２．２～３．８倍，抗菌肽含量为０．１μｇ时即可观察到有明显的抑菌圈，薄层平板法不仅可保证琼脂糖厚度均匀一致，从而提高检测结果的稳定性，而且可由原来的半定量发展为定量测定。此法可广泛应用农业、医药，对各种抗菌物质作微量测定及对不同抗菌物质的抑菌活性进行比较。The commonly used agarose diffusion assay on glass-plate was modified by using a thin-lay-er agarose plate for detection of biological activity of antibacterial polypeptide. By using this method,thesensitivity was increased 2.2～3.8 times.When 0.1 μg antibacterial polypeptide,Shiva l.was loaded to thehole on agarose thin-layer plate,the antibacterial transparent zone could be cleary defined.This method in-sures an even thickness of agarose plate so that the results are reproducible. While previous agarosedif-fusion method on glass-plate can only detect semi-quantitatively,thes method provides a quantitative assayof antibacterial substances.The method is believed to be able to widely used in agricultural and medicalfields for assaying antibacterial compounds and for comparing the biological activity of different antibacteri-al substances.

关 键 词：抗菌肽 抑菌活性 薄层平板 检测 

分 类 号：S482.2[农业科学—农药学]