猪毛水解母液中氨基酸的反相高效液相色谱分析  

Sensitive Determination of Amino Acid Composition of MotherLiquor Prepared by Hydrolyzing PigHair with Reversed-Phase High Performance Liquid Chromatography(HPLC)

作　　者：金燕[1] 沈恩云 金鑫荣[1] 孙科夫 

机构地区：[1]华东理工大学理学院

出　　处：《色谱》1995年第3期220-222,共3页Chinese Journal of Chromatography

摘　　要：猪毛水解母液中氨基酸的反相高效液相色谱分析金燕，沈恩云，金鑫荣，孙科夫（华东理工大学理学院上海２００２３７）１前言近年来，高效液相色谱（ＨＰＬＣ）在复杂的生化分析中的应用日益广泛，成为目前普遍使用的分析氨基酸的方法。由于大多数氨基酸无紫外吸收和荧光发...new method for separation and quantification of amino acids by pre-column derivatization withphenylisothiocyanate(PITC ) and reversed-phase HPLC is described. Seventeen amino acids were separatedin 30 min at room temperature.The method has been successfully applied to the determination of amino acidcomposition in the solution which was prepared by hydrolyzing pig hair and extration of cystine.

关 键 词：氨基酸 猪毛水解 高效液相色谱 

分 类 号：O629.7[理学—有机化学] O652.63[理学—化学]