毛细管气相色谱法测定大白鼠生物膜标本中磷脂脂肪酸的含量  

Determination of Fatty Acids in the Phospholipid of Rat Biological Membrane by Capillary Gas Chromatography(CGC)

作　　者：章满[1] 胡曼玲[1] 唐茂云[1] 周光宇[1] 张文敏[1] 

机构地区：[1]湖南医科大学预防医学系

出　　处：《色谱》1995年第6期415-417,共3页Chinese Journal of Chromatography

基　　金：国家自然科学基金1992年资助项目

摘　　要：用十七烷酸（Ｃ＿（１７：０））作内标，对大白鼠红细胞膜、肝徽粒体膜、心肌细胞线粒体膜等生物细胞膜中磷脂脂肪酸含量进行毛细管气相色谱分析。通过低温差速离心，从大白鼠血、肝及心组织中获得纯净的红细胞膜、肝微粒体膜及心肌细胞线粒体膜。用正己烷萃取去掉可能残存于膜样品中的游离脂肪酸，用氯仿甲醇混合提取液提取膜类脂，以５％三氯化用甲醇溶液加热回流，使磷脂脂肪酸水解并甲酯化，其平均酯化率为９４．５％。对各次加入不同量Ｃ＿（１２：０）→Ｃ＿（２２：６）的回收率为９４．５％～９９．８％。重复性实验中各脂肪酸的平均变异系数ＣＶ为３．９％。方法简便可靠。atty acids in phospholipid of the rat biological membrane,liver microsome and heart mitochondria ofthe rat were determined by CGG by using heptadecanoic acid(C_(17:0))as internal standard(i.s.).By means oflow temperature differential-speed centrifuge pure biological membranes were obtained from blood,liver andheart of the rat.To get rid of free fatty acids which may possibly remain in sample membrane the membranelipids were extracted by using n-hexane and again a mixture of chloroform-methanol.Esterify the acids with5%boron trichloride-methanol.The average esterification rate was 94.5%. Recoveries obtained by addingdifferent amounts of C_(10:0)～C_(22:6) were 94.5%～99.8%. CV of the determination was 3.9%,The methodwas proved to be simple and reliable.

关 键 词：气相色谱 磷脂脂肪酸 大白鼠 生物膜 

分 类 号：Q547[生物学—生物化学] O657.71[理学—分析化学]