PWM诱导的胎儿胸腺细胞钙动员  

Effects of PWM on the Calcium Mobilization of Human Fetal Thymocytes

作　　者：孙永莲[1] 马树俊 尹益民[1] 宗淑珍 吕平[1] 彭大成[1] 

机构地区：[1]北京医科大学免疫学系,北京医科大学第三医院,北京医科大学分析计算中心

出　　处：《上海免疫学杂志》1995年第6期348-351,共4页Shanghai Journal of Immunology

摘　　要：本文用钙离子荧光探针Ｆｌｕｏ－３和ＰＥ－ＣＤ３双标记的方法，通过流式细胞计测定ＰＷＭ对ＣＤ３表达量不同的胎儿胸腺细胞内游离钙浓度的影响。结果表明，ＰＷＭ能迅速诱导胸腺细胞并且主要是ＣＤ３＋细胞钙动员，７０ｍｉｎ后胞内钙基本降至基线水平；Ｈ７、ＣＰＺ和ＥＧＴＡ均能抑制ＰＷＭ诱导的钙动员。Effects of PWM(pokeweed mitogen) on the calcium mobilization of human fetal thymocytes was measured by dual-color analysis using Fluo-3 and phycoerythrin(PE) -conjugated cD3 with nowcytometry.The results showed that: PWM induced a rapid calcium mobilization in thymocytes,mainly in CD3+ cells,and which could be inhibited by H7,CPZ and EGTA.

关 键 词：PWM 胎儿胸腺细胞 钙动员 T细胞 

分 类 号：R392.12[医药卫生—免疫学]