非霍奇金淋巴瘤Ig和TCR_β链基因重排与相关抗原的表达  

Ig AND TCR_β CHAIN GENE REARRANGEMENTS AND CELL SURFACE ANTIGEN EXPRESSION IN NON-HODGKIN'S LYMPHOMA

作　　者：杜豫[1] 顾健人[2] 许良中[1] 蒋惠秋[2] 

机构地区：[1]上海医科大学肿瘤医院病理科 [2]上海市肿瘤研究所

出　　处：《上海医科大学学报》1995年第2期81-84,共4页Journal of Fudan University(Medical Science)

基　　金：国家自然科学基金!(39170340)资助

摘　　要：用１４种抗人白细胞的单克隆抗体（ＭｃＡｂ）与２种多克隆抗体（ＰｃＡｂ）和人类免疫球 蛋白（Ｉｇ）基因簇重链（ＩｇＨ）ＪＨ、Ｃμ、轻链（ＩｇＬ）Ｃκ、Ｏλ以及Ｔ细胞受体β链（ＴＣＲβ）基因作为 探针，以 ＡＢＣ及 Ｓｏｕｔｈｅｒｎ印迹杂交法对１１例非霍奇金恶性淋巴瘤（ＮＨＬ）的抗原表达与 Ｉｇ基因簇、ＴＣＢβ基因重排作了研究。结果显示：抗原表达和基因重排具有非常明确的相关性（Ｐ ＝０．００２）。从ＩｇＬ的κ链重排中还显示了基因的重排早于基因的表达。研究证实基因重排的检测能更敏感地反映ＮＨＬ的亚型以及肿瘤的分化阶段。The expression of differentiation antigens and the rearrangement of Ig and TCRβ genes were studied in 11 cases of NHL by ABC immunoperoxidase method and southern blot hybridization technique. The results demonstrated that there was a definite correlation (P=0.002) between the expression of differentiation antigen and teh structures of Ig and TCRe gene. Ig heavy chain (IgH) rearran- gement was seen in all the 6 cases with B-lineage immunophenotype. The expres- sion of Ig light chain (IgL) was also consisted with the result from southern blot analysis. Furthermore, the kappa chain(Cκ） rearrangement in IgL showed that the gene rearrangement occured earlier than gene expression. Four of the 5 cases with T-lineage immunophenotype had TCRβchain rearrangement. The present study suggsets that the detection of gene rearrangement is able to distinguish the subtype of NHL and the differentiation stage of tumor cells with higher sensitivity.

关 键 词：TCRΒ 基因重排 淋巴瘤 免疫球蛋白 抗原 

分 类 号：R733.402[医药卫生—肿瘤]