血清碱性磷酸酶的凝集素亲和层析在肝脏疾病鉴别诊断中的应用  

APPLICATION OF LECTIN AFFINITY CHROMATOGRAPHY OF SERUM ALKALINE PHOSPHATASE IN THE DIFFERENTIAL DIAGNOSIS OF LIVER DISEASES

作　　者：陈惠黎[1] 吕晴[1] 吕元[1] 

机构地区：[1]上海医科大学卫生部糖复合物重点实验室,华山医院检验科

出　　处：《上海医科大学学报》1995年第5期329-334,共6页Journal of Fudan University(Medical Science)

基　　金：国家自然科学基金;中华医学基金会CMB基金

摘　　要：血清碱性磷酸酶（ＡＬＰ）通过ＷＧＡ亲和层析柱后，可分成不结合的峰Ⅰ和结合的峰Ⅱ两个活力峰，峰Ⅰ为肝型（Ｌ－ＡＬＰ），而峰Ⅱ为骨型（Ｂ－ＡＬＰ）。Ｌ－ＡＬＰ在急性肝炎时明显增高；慢性肝炎或肝癌时轻度上升，而肝硬化时变化不大。如将Ｌ－ＡＬＰ再通过欧蔓陀罗凝集素（ＤＳＡ）亲和层析柱，则正常血清Ｌ－ＡＬＰ完全不被ＤＳＡ结合；急性肝炎的血清Ｌ－ＡＬＰ有部分为弱结合；慢性肝炎和肝硬化除弱结合组分外，出现较多的中度结合组分；而原发性肝癌则出现中度结合组分及特有的强结合组分。这些结果说明肝病越发展成慢性或恶习性或恶性，血清Ｌ－ＡＬＰ和ＤＳＡ的结合力越强。这可应用于临床上鉴别各类不同肝的。肝病血清Ｌ－ＡＬＰ结合于ＤＳＡ增多的原因主要是Ｎ－连接型糖链的无线数增加，后者尚可从天线末端唾液酸增多以及强结合组分不被ＣｏｎＡ结合而获得证明。Serum alkaline phosphatase(ALP) were fractionated by WGA affinity chromatography into two active peaks unbinding peak Ⅰand binding peak Ⅱ,being live type(L-ALP) and bone type(B-ALP) ALP respectively. L-ALP was significantly increased in the sera of acute hepatitis, slightly elevated in chronic hepatitis, but not obviously changed in liver cirrhosis.If- ALP was snbjected to DSA affinity chromatography, it was found that L-ALP from normal sera were totally unbound to DSA,but those from acute hepatitis were partially bound with weak affinity, Sera of chronic hepatitis and live cirrhosis, in addition to weakly binding portion,contained a significant binding portion with medium affinity, and sera of primary liver cancer contained a binding portion with medium affinity and another specific strong binding fraction. These results suggested that the more progressive the liver diseases changed to ward chronic or malignant stage, the stronger the binding of L-ALP to DSA,and this may be applied to differential diagnosis of various liver diseases in clinical medicine,The main reason of increased binding of serum L-ALP to DSA was the increased antennary number of N-linked sngar chains in L-ALP,which was also proved by the increase of antennary terminal sialic acids and the unbinding of DSA binding fraction to Con A.

关 键 词：碱性磷酸酶 肝脏疾病 凝集素 亲和层析 

分 类 号：R575.04[医药卫生—消化系统]