羟自由基对培养细胞损伤作用的实验观察  

Cell Damage in Chinese Hamster V79 CellsTreated With Hydrogen Peroxide.

作　　者：汪德清[1] 沈文梅[1] 田亚平[1] 董振南[1] 刘国廉[1] 孙仲怡[1] 杨素霞[1] 周淑珍[1] 

机构地区：[1]解放军总医院生化科,军事医学科学院放射医学研究所

出　　处：《生物化学与生物物理进展》1995年第3期278-281,共4页Progress In Biochemistry and Biophysics

摘　　要：将不同浓度的Ｈ＿２Ｏ＿２与Ｖ７９细胞共同孵育２４、４８ｈ后，结果显示ＳＯＤ活性下降，ＬＰＯ含量增加，细胞内ＧＰＴ、ＧＯＴ、ＬＤＨ和ＣＰＫ酶活性升高，其变化程度与培养时间和Ｈ＿２Ｏ＿２浓度成正比．When Cmnese Hamster V79 cellswere incubated with H_2O_2 of different concen-tration for 24h and 48h,different degree dam-ages in cells have been found.The activity ofSOD decreased and lipid peroxides contents in-creased in cultured cells.It is also shown thatthe activies of metabolites,such as GPT,GOT,LDH and CPK,are higher than thosein control,is in direct proportion to the H_2O_2concentration and the incubation time.

关 键 词：羟自由基 细胞损伤 过氧化脂质 

分 类 号：R361.3[医药卫生—病理学]