超临界CO_2技术萃取蛋黄磷脂  

The Extraction of Yolk Phospholipid by theTechnique of Supercritical Fluid CO_2 Extrac-tion.

作　　者：赖炳森[1] 毛中兴[1] 沈晓京[1] 孙树秦 

机构地区：[1]解放军北京医学高等专科学校生物化学教研室

出　　处：《生物化学与生物物理进展》1995年第4期366-370,共5页Progress In Biochemistry and Biophysics

基　　金：全军"八五"医药卫生科研基金

摘　　要：采用新型物理分离技术──超临界ＣＯ＿２萃取法，提取天然蛋黄粉中的磷脂．在４０ＭＰａ，先去除蛋黄粉中甘油三酯和胆固醇，再萃取磷脂．结果显示，磷脂纯度为９５％，Ｎ／Ｐ比值为１．００３，λ＿（ｍａｘ）＝２１４ｎｍ，薄层层析显示磷脂着色点清晰，并去除了绝大部分甘油三酯和胆固醇。此法操作简单、产品质量高、安全和不污染环境，还可得到天然纯蛋黄油和蛋白。Phospholipid(PL)has higher med-ical and nutritional values and widely appliedin medical,food,Chemical industry field etc.The natural PL of yolk powder was extractedby supercritical fluid CO_2 extraction(SFE-CO_2)technique. The phosphorus,nitrogenand PL contents,the N/P molar ratio,thetriglyceride and cholesterol of the extractionwere measured by means of biochemicalmethod,thin layer chromatography,andspectral analysis. The results showed that ex-tracted yolk PL is 95%in purity and its N/Pmolar ratio is 1.003.So the SFE-CO_2technique can be used to obtain yolk PL withhigher purity and quality.

关 键 词：磷脂 蛋黄 超临界CO2萃取 

分 类 号：Q545.03[生物学—生物化学]