酵母PH081在酸性磷酸酯酶基因表达调控中的作用  

The Function of PHO81 in the Regulatory System of YeastAcid Phosphatase Gene Expression

作　　者：吴健生[1] 陈金莲[1] 敖世洲[1] 

机构地区：[1]中国科学院上海生物化学研究所分子生物学国家重点实验室

出　　处：《生物化学与生物物理学报》1995年第3期241-246,共6页

摘　　要：利用酵母ＰＨＯ８１与大肠杆菌产β－半乳糖苷酶的融合基因，系统地研究了ＰＨＯ８１基因在酵母酸性磷酸醋酶的不同调控因子的单缺失株和双缺失株中的表达规律，它与酸性磷酸酯酶基因ＰＨＯ５和ＰＨＯ１１的控制机制相似。构建了ＡＤＨ１启动子控制下ＰＨＯ８１表达质粒。在ＰＨＯ８１在细胞内组成型表达时，酸性磷酸酯酶基因的表达仍受无机磷的控制，揭示ＰＨＯ８１蛋白可能在不同浓度无机磷条件发生变构。ＰＨＯ８１在酸性磷酸酯酶基因表达系统中是一个中介因子。在此基础提出了一个酸性磷酸酯酶基因调控的分子模型。Using the fusion gene of PHO81 with β-galactosldase, we studied the expressions of the PHO81 gene in the single mutant and double mutant strains of various regulatory factors of the yeast acid phosphatase genes PHO5 and PHO11, and found that the regulation of the PHO81 gene was similar to that of the PHO5 and PHO11. A constitutive expression vector of PHO81 gene under the control of yeast ADH1 promoter was constructed and transfoTmed to the mutant strain of PHO81 gene. When the expression of PHO81 was constitutive, the expression of yeast acid phophatase gene was still repressed under the high-Pi conditions, suggesting that PHO81 gene product might change its conformation with the change of the concentration of inorganic phosphate. PHO81 was a medistor in the PHO system. A molecular model of the expression of the yeast acid phosphatase was proposed.

关 键 词：酵母 PHO81基因 基因表达 体内同源重组 

分 类 号：Q939.506[生物学—微生物学]