HL－60细胞诱导分化过程中TPK和PTPP活力的时空改变  

Temporal & Spatial Alternations of TPK and PTPP Activities During the Induced Differentiation of Human Promyelotic Leukemia Cells (HL-60)

作　　者：白龙川[1] 颜卉君[1] 吴国利 

机构地区：[1]北京师范大学分子生物学及生物化学研究室

出　　处：《生物化学杂志》1995年第3期333-337,共5页

基　　金：国家自然科学基金

摘　　要：对ＲＡ、ＨＨＴ和ＷＰ_（８５２）诱导ＨＬ－６０细胞分化过程中胞浆和膜溶脱部分中的ＴＰＫ和ＰＴＰＰ活力变化进行了研究．结果表明，在诱导早期，ＴＰＫ的活力就有明显的波动变化；随着诱导时间的延长，胞浆部分ＴＰＫ活力下降，膜溶脱部分的ＴＰＫ活力则升高．诱导后，胞浆部分和膜溶脱部分的ＰＴＰＰ活力均明显升高。与ＴＰＫ活力变化相比较，ＰＴＰＰ变化幅度比ＴＰＫ的大。Alternations of TPK and PTPP activities in cytosol and membrane fractions were studied during the differentiation of HL-60 cells induced by RA. HHT and WB_(852). It was found that the activity of TPK showed a distinct fluctuation in the early stage of differentiation and with the elongation of differentiation,it declined in cytosol fraction and increased in membrane fraction. It was also found that the activity of PTPP increased distinctly both in cytosol and memerane fractions. The range of increase was greater than the range in change of TPK activity.

关 键 词：人早幼粒白血病 蛋白激酶 TPK 白血病 细胞分化 

分 类 号：R733.702[医药卫生—肿瘤]