反胶束萃取胰蛋白酶的研究  

Extraction of Trypsin Using Reversed Micelles

作　　者：陆强[1] 李宽宏[1] 施亚钧[1] 

机构地区：[1]华东理工大学化学工程研究所

出　　处：《生物技术》1995年第2期13-15,18,共4页Biotechnology

基　　金：国家自然科学基金

摘　　要：本文以含有反胶束的有机溶剂作为萃取剂，进行了将胰蛋白酶从水相传入有机相，再从有机相传入另一水相的研究。结果表明：影响萃取率的主要因素为水相出值、离子强度和种类，以及反胶束溶液中表面活性剂浓度等。在适宜的条件下，酶的单级萃取和反萃取率都很高，显示了良好的工业应用前景．Trypsin transport between an aqueous phase and an organic phase containing reversed micelles was studied. The partitioning behaviour of the enzyme depends on the parameters ofbinary phases (for example: the PH value, the concentration and species of ion in aqueousphase, the content of surfactant in reversed micellar solution). When the operation runs under optimized conditions, one extraction--stripping stage efficiency is high. It shows that theextraction of enzymes using reversed micelles is a promising technique for application at thedownstream biotechnological processes.

关 键 词：反胶束 萃取 胰蛋白酶 

分 类 号：Q556.903[生物学—生物化学]