恶性肿瘤癌旁组织DNA倍体的流式细胞测量术分析  

FlowCytometri cStudyonCellularDNAContent Of paracancer Tissue in Malignant Tumor

作　　者：周振英[1] 张军妮[1] 蒋明[1] 顾平[1] 孙克华 

机构地区：[1]江苏省肿瘤防治研究所,南京军区总医院

出　　处：《实用癌症杂志》1995年第2期82-85,共4页The Practical Journal of Cancer

摘　　要：作者采用流式细胞测量术对７种常见恶性肿瘤２８２例癌旁组织进行了ＤＮＡ含量测定。结果表明，癌旁组织异倍体检出率为４４．７％。在各种癌旁组织的异倍体类型中，以非整倍体（ＡＮ）为主，占异倍体病例的８１．０％（１０２／１２６例）．癌旁组织ＤＮＡ指数（ＤＩ）显著低于癌中心灶，但其中异倍体病例的ＤＩ值与癌中心灶十分接近。当癌中心灶为近三倍体时，其癌旁组织异倍体检出率最高。癌旁组织与癌中心灶ＤＮＡ干系的一致率为７３．２％。在二倍体肿瘤中，有２２．４％病例的癌旁组织出现了异倍体细胞。在各种肿瘤中，肝癌、食管癌、脑肿瘤和贲门癌的癌旁组织各项指标变化最明显。DNA content was measured on 282 samples of para- cancer tissue with flow cytometry.The detecablerate of abnormal ploid was 44.7%in para-cancer tissues.The percen tage of aneuploid(AN)was the highest in allabnormal ploidy types of different para-cancer tissues. It was 81.0%(102/126 cases)in abnormal ploidy cases. DNAindex(DI)of para- cancer tissue was markedly lower than that of cancer nests,while there is no significant differancebetween DI val ue of abnormal ploidy cases in para- ca ncer tissue and cancer nests. When cancer nests were near-triploidy cells,the percentage of abnormal ploidy was highest in its para- cancer tiSsue. The rate of consistency of DNAstem between cancer nest and para- cancer tissue was 73.2%. There were 22.4%cases of abnormal ploidy in diploidytumor petients. The changes of FCM targets were remarkable in hepatocellular cancer , esophagus cancer,brain tumorandcar diacancer.

关 键 词：恶性肿瘤 癌旁组织 DNA 流式细胞术 

分 类 号：R730.2[医药卫生—肿瘤]