杜氏利什曼原虫基因文库的构建及SSU　rDNA基因的克隆和筛选  

CONSTRUCTION OF THE GENOMIC LIBRARY AND CLONING, SCREENING OF rDNA OF LEISHMANIA DONOVANI

作　　者：陈建平[1] 杨文天[1] 胡孝素[1] 

机构地区：[1]华西医科大学寄生虫学研究室

出　　处：《实用寄生虫病杂志》1995年第3期97-99,共3页Journal of Practical Parasitic Diseases

基　　金：纽约中华医学基金;国家教委博士学科点专项基金

摘　　要：采用新型载体（ＬａｍｂｄａＧＥＭＲ－１１ｖｅｃｔｏｒ系统）完成了杜氏利什曼原虫四川人分离株（简称Ｌ．ｄ．四川人株）的基因组ＤＮＡ大片段克隆。重组噬菌体总数为１．５×１０６。采用地高辛标记的Ｌ．ｄ．四川人株ＳＳＵｒＤＮＡ扩增产物探针筛选文库，获得３个含有Ｌ．ｄ．四川人株ｒＤＮＡ插入片段的重组噬菌体克隆，为今后的亚克隆和ＳＳＵｒＤＮＡ可变区及间隔区结构和功能研究打下了基础。Leishmanai donovani (L. d. ) (MHOM/CN/86/SC6) genomic library has been constructed by advanced Lambda GEMR-11 vector in our laboratory. L. d. promastigotes genomic DNA was partially digested with restriction enzyme Sau 3A I, ligated to Lambda GEMR-11 arms and packaged is vitro, resulting in 1. 5×106 phase. After screened with L. d. SSU rDNA amplified products probe, three positive recombinant phage colonies have been got. The result will lay good foundation for further study of subcloning, sequencing, structure and function in the future.

关 键 词：杜氏利什曼原虫 SSU RDNA 克隆 筛选 基因文库 

分 类 号：R382.22[医药卫生—医学寄生虫学]