催产时黄鳝性类固醇激素含量变化的研究  

CHANGES OF SEX STEROIDS IN MONOPTERUS ALBUS DURING ITS SPAWNING INDUCED WITH INJECTION OF LHRH-A

作　　者：周定刚[1] 郑维明[1] 张大祥[1] 

机构地区：[1]四川农业大学

出　　处：《水生生物学报》1995年第4期311-316,共6页Acta Hydrobiologica Sinica

基　　金：四川省科委应用基础理论资助项目

摘　　要：本文首次报道哺乳动物促黄体生成素释放激素类似物（ＬＨＲＨ－Ａ）诱导黄鳝排卵过程中，血浆雌二醇（Ｅ＿２）、孕酮（Ｐ）和睾酮（Ｔ）含量变化的规律。根据１７２尾黄鳝血样放射免疫测定结果，试验组雌鱼干排卵前相继出现Ｅ＿２和Ｐ浓度高峰（Ｐ＜０．０１）；雄鱼于催产后２４ｈ出现Ｔ浓度峰值（Ｐ＜０．０１）；试验组雌鱼的Ｔ含量与对照组无显著差异（Ｐ＞０．０５）。在对照组，雄鱼的Ｅ＿２和Ｐ浓度及雄鱼的Ｔ含量差异不显著（Ｐ＞０．０５）。This paper reports, for the first time, the changes in concentrations of estra-diol(E_2), progesterone(P)and testosterone(T)in serum of Monoptcrus albus du-ring the spawning induced with injection of LHRH-A(mammalian luteinizing hor-mone-releasing hOrmone analogues). The female fish injected with LHRH-A(0.3μg/gram body weight) exhibited a significant increase(P<0.05) in serum E_2 con-tent seven hrs after injection(A.I.).The E_2 content reached its peak level 24 hrsA.I. The progesterone content showed also a similar increase: a significant rise inserum P content was observed 24 hrs A.I.(P<0.05)and a more significant rise 48hrs A.I(P<0.01). However, during the course of ovulation concentrations of E_2and P decreased gradually towards their levels observed before injection. Changesin testosterone concentration showed a somewhat different pattern in serum of themale fish injected with LHRH-A: the peak concentration level of T was detected24 hrs A.I when spermiation took place and the T content decreased to its originallevel after spermiation. The theoretical basis was therefore presented on the useof sex steroids as an agent to induce spawning of M. albus.

关 键 词：黄鳝 诱导排卵 类固醇激素 催产 

分 类 号：Q959.482[生物学—动物学]