真养产碱杆菌突变株65－7产羟基丁酸与羟基戊酸共聚物的研究  

STUDIES ON COPOLYESTR CONSISTING OF 3- HYDROXYBUTYRATE AND 3-HYDROXYVALER- ATE BY THE MUTANT 65-7 OF ALCALIGENES EXTROPHUS

作　　者：翁维琦[1] 易祖华[1] 黄和容[1] 陈琦[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《微生物学通报》1995年第5期271-275,共5页Microbiology China

摘　　要：研究了真养产碱杆菌突变株６５－７，以葡萄糖为主原料，添加丙酸或戊酸，采用二步发酵积累共聚物聚β－羟基丁酸－β－羟基戊酸（ＰＨＢＶ）。摇瓶总发酵时间为５０ｈ，细胞干重达７－１１ｇ／Ｌ，共聚物含量占细胞干重的７０％以上，其中β－羟基戊酸（３ＨＶ）含量占ＰＨＢＶ的１０－７２％，主要取决于不同碳源的组成，丙酸和戊酸对ＨＶ的转化率分别为０．４１－０．６３ｇＨＶ／ｇ丙酸和０．４０－０．７４ｇＨＶ／ｇ戊酸，制得的ＰＨＢＶ产品纯度９９％以上，分子量６．９×１０￣５。By two-stage fermentation,the synthesis of the copolymer of 3-hydroxybu-tyrate(3HB) and 3-hydroxyvalerate(3HV),PHBV,by the mutant 65-7 of Alcaligeneseutrophus from glucose and propionic(or pentanoic) acid was studied.For 50 hours,thetotal cell concentration in shake-flask was 7-10g/L,and the PHBV content reached upto70%of the dry cell weight.The 3HV fraction in the copolymers was 10-74%,dependingon the composition of carbon suhstrates supplied. The convertion from propionic and penta-noic acid to 3HV was 0.41-0.63 and 0.40-0.74 wt/wt, respectively.The molecular weightof PHBV isolated from the biomass was 6.9× 10￣5 with purity more than 99%.

关 键 词：真养产碱杆菌 突变株65-7 羟基丁酸 羟基戊酸 

分 类 号：TQ324.9[化学工程—合成树脂塑料工业]