脑啡肽对小鼠神经母细胞瘤细胞脂褐素影响的实验研究  

AN EXPERIMENTAL ANALYSIS OF THE EFFECT OF LEUCINE ENKEPHALIN ON LIPOFUSCIN OF MOUSE NEUROBLASTOMA A_2 CELLS

作　　者：孙晓江[1] 蔡琰[1] 苏敏[1] 王珺[1] 张毅[1] 邓锦波[1] 孙九伶[2] 高彦军[2] 

机构地区：[1]上海第二医科大学附属仁济医院神经生物学实验室,上海200001 [2]承德医学院附属医院神经生物学实验室

出　　处：《细胞生物学杂志》1995年第4期189-192,共4页Chinese Journal of Cell Biology

基　　金：上海高等院校科学发展基金;河北省自然科学基金

摘　　要：本文应用小鼠神经母细胞瘤细胞A_2(NB-A_2)无血清培养建立的神经细胞老化实验研究模型,以显微荧光分光光度术测定单个细胞内脂褐素荧光值作为细胞老化指标,观察了脑啡肽(LE)对NBA_2细胞内脂褐素荧光值的影响。结果表明LE可显著降低细胞内的脂褐素荧光值(P<0.01)。提示LE可延缓NBA_2细胞的老化进程。Serum-free cultures of mouse neuroblastoma A2 (NBA2) cells were used as the experimental model for the study of neuronal aging, with microfluorometry of the autofluorescencc of lipofuscin as the index of neuronal aging. The results showed that fluorescent value (F.V.) of lipofuscin increased progressively with prolonging of culture days. The results implied that NBA2 cells in scrum-free culture change from division and reproduction into differentiation and maturation, reflecting the process of neuronal aging.After addition of Icucinc cnkephalin (LE) separately into the culture medium, the results showed that F. V. of lipofuscin was decreased in 5,10 and 15 day treatment group (P<0.01). The result suggested that LE had the property to retard the accumulation of lipofuscin and produced effect in the direction of delaying NBA2 cells iging.

关 键 词：神经母细胞瘤 细胞 脂褐素 脑非肽 肿瘤 

分 类 号：R730.264[医药卫生—肿瘤] Q514.3[医药卫生—临床医学]