聚合酶链反应检测宫颈癌的前瞻性研究  

The Prospective Study on Cervical Carcinoma by PCR

作　　者：陈诵芬[1] 杨道理[1] 王宝成[1] 克丙申[1] 单乐媛 谢华[1] 

机构地区：[1]山东济南军区总医院妇产科

出　　处：《现代诊断与治疗》1995年第1期11-13,共3页Modern Diagnosis and Treatment

摘　　要：采用聚合酶链反应（ＰＣＲ）对宫颈癌、宫颈糜烂、正常宫颈组织各５０例进行了人乳头瘤病毒（ＨＰＶ）的检查。结果提示从宫颈正常组织到癌，ＨＰＶ＿１６、ＨＰＶ＿１８的检出率逐渐升高，尤其ＨＰＶ＿１６呈明显梯度关系，宫颈癌检出率显著高于正常组织及炎症组织（Ｐ＜０．０１）。说明宫颈组织自身ＨＰＶ＿１６的感染率与宫颈癌的发生密切相关，与ＨＰＶ＿１８有一定的联系。通过癌旁组织，宫颈糜烂组织及正常宫颈刮片检测出不同的ＨＰＶ＿１６阳性率的分析，说明ＨＰＶ感染是癌发前的重要因素，指出凡检出ＨＰＶ＿１６阳性者应视为“高危人群”给予长期密切监护治疗。The HPV in 150 samples including 50cases of cervical carcinoma,50 cases of cervicalerosin and 50 cases of normal cervical tissue werestudied by polymerase chain reaction(PCR),to in-vestigate the relationship between cervical carcinomaand HPV infection. The PCR results showed that the HPV_16 infec-tion rate in cervical carcinoma was statistically morehigh than that in cervical erosion and normal tissue(P<0.01).It suggested that the HPV_16 infection may be themain cause for cervical carcinoma,and HPV_18 has the same ex- pression as HPV_16.To analyse the different HPV_16 infectionrate from the tissues of pericancer,cervical erosion and normalcervical smear has suggested that the HPV infection may bethe important factor to cause precancer. The cases of HPV_16positive should be treated as a high-risk population and shouldbe long-term closely monitor and therapy.

关 键 词：聚合酶链反应 宫颈癌 人乳头瘤病毒 

分 类 号：R737.330.3[医药卫生—肿瘤]