单纯疱疹Ⅱ型病毒感染对培养的猪主动脉平滑肌细胞形态和LDH释放的影响  

Effects of Herpes Simplex Virus Type Ⅱ Infection on Morphology and the Release of Lactate Dehydrogenase in Porcine Aortic Smooth Muscle Cells in Culture

作　　者：徐惠芳[1] 杨小毅[1] 黄红林[1] 

机构地区：[1]衡阳医学院

出　　处：《心肺血管病杂志》1995年第1期42-43,共2页Journal of Cardiovascular and Pulmonary Diseases

摘　　要：病毒感染与动脉粥样硬化之间的关系近年来逐步受到人们重视，本文以培养的猪主动脉平滑肌细胞（ＳＭＣ）为对象，观察了单纯疱疹Ⅱ型病毒（ＨＳＶ－Ⅱ）感染对细胞乳酸脱氢酶（ＬＤＨ）释放及形态的影响。结果显示，ＨＳＶ─Ⅱ感染主动脉平滑肌细胞４８─７２ｈ后，会引起ＳＭＣ出现细胞病变，并且呈滴度依赖性释放乳酸脱氢酶，在高滴度时甚至呈现溶细胞病变。提示长期潜伏存在的疱疹病毒可能作为一种局部损害因子。造成动脉壁平滑肌细胞的损伤。Herpes viruses have been implicated as etiologic factors in the pathogenesis of human atherosclerosis.In this paper,porcine aortic smooth muscle cells(SMC) in culture were infected with herpes simplex virus type Ⅱ(HSV-Ⅱ).The cytopathic effect(CPE) was observed for 48～72h after infection.There was a significant increase in the release of lactate dehydrogenase(LDH) in HSV-Ⅱ-infected porcine SMC.The results suggested that ubiquitious herper viruses may be one of the injurious agents for inciting atherosclerosis.

关 键 词：单纯疱疹病毒 乳酸脱氢酶 主动脉 平滑肌细胞 

分 类 号：R363.14[医药卫生—病理学] R373[医药卫生—基础医学]