海南粗榧新碱衍生物HH07A的抗肿瘤作用  

STUDIES ON THE ANTICANCER EFFECT OF HH07A,A DERIVATIVE OF HAINANENSINE

作　　者：叶玉梅[1] 徐承熊[1] 孙润华 郭积玉[1] 崔光建 

机构地区：[1]中国医学科学院,中国协和医科大学药物研究所

出　　处：《药学学报》1995年第1期12-16,共5页Acta Pharmaceutica Sinica

摘　　要：用细胞生长曲线测定法及软琼脂集落形成分析法研究了ＨＨ０７Ａ对几种肿瘤及正常细胞生长的影响。结果表明，１．５ｕｇ·ｍｌ－１及３μｇ·ｍｌ－１ＨＨ０７Ａ能分别明显抑制Ｌ１２１０和ＨＬ－６０细胞的生长。３种肿瘤细胞对ＨＨ０７Ａ的敏感性依次为Ｌ１２１０＞ＫＢ＞ＨＬ－６０，而正常小鼠粒系祖细胞ＧＭ－ＣＰＣ对药物的敏感性则低于前三者，且ＨＨ０７Ａ３．５μｇ·ｍｌ－１对ＨＬ－６０细胞无分化诱导作用。ＨＨ０７Ａ对腹水型Ｌ１２１０白血病小鼠、Ｓ１８０小鼠均有较明显的治疗作用，使Ｌ１２１０荷瘤小鼠、Ｓ１８０荷瘤小鼠存活时间延长。也能抑制Ｓ１８０实体瘤的生长。The effect of HH07A on the growth of tumor cells in vitro was jnvestigated usingthe techniques of cell growth curve determination and soft-agar colony-forming assay.The resultshowed that HH07A inhibited the growth of L1210 cells and HL-60 cells at a concentration of 1.5μg· ml-1 and 4.0μg· ml-1,respectively.Among the cells tested,L1210 cells were shown to be themost sensitive,followed by KB cells and HL-60 cells(with IC50 of 2.29,4.13 and 4.36μg· ml-1,respectively). Normal mouse granulocyte-macrophage progenitor cells(GM-CFC)were less sensitiveto the drug(with IC50 of 11.15μg·ml-1)as compared with the tumor cells.As they were exposed toHH07A 3.5μg· ml-1 for 5 days,HL-60 cells did not show NBT reductive ability. Intraperitonealinjection of HH07A exerted inhibitory effect on the ascitic tumors of L1210 and S180 in mice.oral orip administration of HH07A also showed some effect on S180 solid tumors in miee.

关 键 词：海南粗榧 克隆原细胞 抗肿瘤作用 生物碱 

分 类 号：R285.5[医药卫生—中药学] R979.1[医药卫生—中医学]