血管紧张素Ⅱ及牛磺酸对体外培养乳鼠肥大心肌细胞心律失常发生的作用  

EFFECT OF ANGIOTENSIN AND TAURINE ON ARRHYTHMIA IN CULTURED NEONATAL RAT HYPERTROPHIC HEART MYOCYTES

作　　者：陶亮[1] 王洪新[1] 饶曼人[1] 

机构地区：[1]南京医科大学心血管药理研究室

出　　处：《药学学报》1995年第5期326-330,共5页Acta Pharmaceutica Sinica

摘　　要：在培养７２ｈ的乳鼠心肌细胞，血管紧张素Ⅱ（ａｎｇｉｏｔｅｎｓｉｎⅡ，ＡｇｎⅡ）１，１０，１００和１０００ｎｍｏｌ·Ｌ－１时，可浓度依赖性地增加细胞搏动频率；而牛磺酸５，１０和２０ｍｍｏｌ·Ｌ－１不影响心肌细胞搏动频率，但能浓度依赖性地拮抗ＡｎｇⅡ１００ｎｍｏｌ·Ｌ－１加快心肌细胞搏动频率的作用。ＡｎｇⅡ１００ｎｍｏｌ·Ｌ－１连续作用７ｄ引起的培养乳鼠肥大心肌细胞搏动频率加快，动作电位ＡＰＡ升高，ＡＰＤ５０和ＡＰＤ９０延长，ＳＣＬ缩短；哇巴因５０ｎｍｏｌ·Ｌ－１诱发的心肌细胞搏动节律失常发生率高于常规培养组。牛磺酸２０ｍｍｏｌ·Ｌ－１可抑制ＡｎｇⅡ引起的上述改变，并降低哇巴因诱发的心律失常发生率。Angiotensin (Ang)Ⅱ(1,10,100 and 1000nmol·L-1)was found to increasespontaneous contractile frequency dose-dependently in neonatal rat cardiac myocytes cultured for 3 d.After exposure to Ang Ⅱ(100 nmol·L-1)for 7 d,neonatal rat heart cells became hypertrophy withincreased frequency ,elevated APA ,prolonged ADP50 and ADP90 ,and shortened SCL.Addition ofouabian(Oua) 50 nmol·L-1 to the hypertrophic myocytes caused more frequent arrhythmia.Taurine(20 mmol·L-1)was shown to inhibit these changes induced by Ang Ⅱ.These results suggest thatAng Ⅱ can increase autorhythmicity as well as sensibility to Oua in cultured catdiac myocytes.Theseeffects might be related to the promotion of Ca2+ influx.

关 键 词：血管紧张素Ⅱ 牛磺酸 动作电位 心律失常 

分 类 号：R541.705[医药卫生—心血管疾病] R965.1[医药卫生—内科学]