一个恢复力受单基因控制的水稻CMS育性回复突变体  

A Single Gene Controlled Fertility-restoring Revertant from Cytoplasmic Male Sterile Rice

作　　者：沈毓渭[1] 蔡其华[1] 高明尉[1] 

机构地区：[1]浙江农业大学原子核农业科学研究所

出　　处：《Acta Genetica Sinica》1995年第6期437-444,共8页

摘　　要：利用￣（６０）Ｃｏ－γ射线对具有印尼水田谷细胞质的籼稻细胞质雄性不育系Ⅱ－３２Ａ干种子进行诱变处理，获得了一育性回复突变体Ｔ２４。育性基因未纯合的突变体分离出可育株和完全不育株，比例为３∶１；其与Ⅱ－３２Ａ和珍汕９７Ａ测交，Ｆ１代分离出１∶１的可育株和不育株。育性稳定株系与Ⅱ－３２Ａ和珍汕９７Ａ杂交，Ｆ２分离成３∶１的可育株和不育株。表明其育性回复是由一对基因显性突变所致。这一突变体对不育系的育性恢复机制不同于明恢６３、２０９６４等恢复系，后者表现为两对显性恢复基因作用。未观察到Ｔ２４与亲本Ⅱ－３２Ａ除育性以外的其他性状的差异，因而两者构成育性恢复基因的近等基因系。本文还对不育系育性回复类型和Ｔ２４的理论意义与育种价值进行了讨论。A fertility revertant T24 from cytoplasmic male sterile(CMS)rice line Ⅱ-32A was induced by dry seed irradiation with 60Co-γrays.The revertant,when still heterogenous,segregated into male fertile and complete male sterile plants in a ratio of 3∶1.Testcrosses of the heterogenous revertant with Ⅱ-32A and Zhenshan 97A displaied 1∶1 segeragation between fertile plants and sterile plants.Besides,the F2 hybrids between Ⅱ-32A and Zhenshan 97A and the stabilized revertant line showed 3∶1 segregation between fertile and male sterile plants. These indicate that the fertility reversion was resulted from the dominant mutation of the fertility gene in CMS line.The revertant might differ in restoring mechanism from other restorer lines,such as Minghui 63 and 20964, which behave in a pattern of two pairs of restoring genes.T24 and its parent Ⅱ-32A menifested no difference except in fertility,therefore,they could be considered as near isogenic lines of fertility-restoring gene.The potential usefulness in study on CMS mechanism and breeding value of the revertant were also discussed.

关 键 词：水稻 细胞质雄性不育 育性回复突变 单基因 

分 类 号：S511.103.5[农业科学—作物学]