利福霉素生产菌产生钝化Rif　SV物质的分离及性质研究  

Isolation and properties or Rifamycin SV-inactivation substance produced by Rif SV-pro-ductive strain

作　　者：吴文芳[1] 张成刚[1] 吕安国[1] 张立新[1] 胡江春[1] 曾青[1] 

机构地区：[1]中国科学院沈阳应用生态研究所

出　　处：《应用生态学报》1995年第3期282-286,共5页Chinese Journal of Applied Ecology

基　　金：中国科学院重点资助

摘　　要：利福霉素ＳＶ（简称ＲｉｆＳＶ）生产菌──地中海拟无枝菌酸菌在生物合成ＲｉｆＳＶ过程中，产生一种能钝化自身产物（ＲｉｆＳＶ）的物质．实验证实，该物质是由谷氨酸、天冬氨酸、赖氨酸及缬氨酸等１４种常见氨基酸组成的蛋白质．分子量（ＭＷ）约２．５×１０４Ｄ，等电点（ＰＩ）为５．７—６．１．在１００℃下加热１０ｍｉｎ，其活性丧失．作用于ＲｉｆＳＶ的最适ｐＨ值范围为７．４—８．６，最适温度为２９℃，初步证实该物质是一种酶（暂称利福霉素钝化酶）The productive strain(A￣ofatoprts nditerranri) produces a substance for inactivating self-product (Rif SV) in the course of Rif SV-biosynthesis.The experiments demonstrate that it is a protein consisting of 14 kinds of common amino acids including Gin, Asp,Lys, Val and so on. Its molecular weight (MW) is approximately 2.5×104D and its isoelectric point (PI) is 5. 7-6. 1. After heated at 100C for 10 min,its activity is disappeared. The optimum PH rang6 for reacting on Rif SV is 7. 4-8. 6 and the optimum temperature is 29℃.It is preliminarily identified as a enzyme (temporarily designated as Rifamycin SV-inactivation enzyme).

关 键 词：利福霉素SV 利福霉素钝化酶 发酵 抗生素生产菌 

分 类 号：TQ927[轻工技术与工程—发酵工程]