应用昆虫杆状病毒载体在昆虫细胞中表达乙肝表面抗原  

Expression of hepatitis B virus surface antigen in insect cells using a baculovirus vector

作　　者：周耐明[1] 宓怡德[1] 金伟[1] 李载平[1] 吴祥甫[1] 

机构地区：[1]浙江农业大学蚕学系,中国科学院上海生物化学研究所

出　　处：《浙江农业大学学报》1995年第1期81-84,共4页

摘　　要：人乙型肝炎病毒（ａｄｒ亚型）表面抗原Ｓ基因克隆到ＰｕＡＣ－５苜蓿尺蠖核型多角体病毒转移载体上，所构建的重组转移载体质粒与野生型ＡｃＮＰＶＤＮＡ共转染Ｓｆ－２１细胞，经空斑法筛选和纯化，得到了含ＨＢＶＳ基因的重组病毒ＡｃＮＰＶＳ．用放射免疫法测定了ＡｃＮＰＶＳ感染的Ｓｆ－２１细胞及其培养液中的ＨＢｓＡｇ，总表达量为１．２２μｇ／１０￣６细胞．The S gene sequence from HBV(adr sub-type)was inserted into a Autographa cali-fornica nuclear polyhedrosis virus(AcNPV ) vector PuAC 5 to construct a recombinant transfervector plasmid PuACHS-5. The Sf-21 cells were co-transfected with PuACHS-5 DNA and wild-type Ac NPV DNA.Arecombinant virus AcNPVS was selected and purified by plaque assay. Thelevel of HBsAg expression in Sf-21 ceIls infected with recombinant virus AcNPVS was estimated atatotal of l.22 t1gl106 cells by radioimmunoassay.

关 键 词：乙型肝炎病毒 表面抗原 昆虫细胞 基因表达 

分 类 号：R373.2[医药卫生—病原生物学] Q962[医药卫生—基础医学]