PP_（333）对马铃薯试管苗生长和长期保存的影响  

The effects of paclobutrazol on in vitro growth and long-term preservation of potato seedlings

作　　者：裘文达 李方[1] 秦文清[1] 陈昆松[1] 

机构地区：[1]浙江农业大学园艺系,浙江医科大学预防科学系

出　　处：《浙江农业大学学报》1995年第3期252-256,共5页

基　　金：浙江省科委资助

摘　　要：研究了自然温度与恒温培养条件下，不同ＰＰ_（３３３）浓度处理对马铃薯试管苗生长和长期保存的影响，结果表明，自然温度培养对马铃薯试管苗延缓生长、延长保存时间、提高存活率更有利，存活率可达１００％，还有促进试管苗结薯的作用。随ＰＰ_（３３３）使用浓度的增加，抑制试管苗生长的作用也增强，保存时间也相应延长。１０ｍｇ／Ｌ处理经３００天培养后，株高几乎与原接种时一样，经ＰＰ_（３３３）处理的试管苗比对照矮壮、叶数增多、根数少而粗短，存活率高。试管苗继代保存期由１年转管４－５次，延长到１－１．５年转管１次。当转入恢复生长的培养基１０天后，就能恢复至正常生长状态。The experiment was conducted to study the effects of different concentrations of PP_(333) on in vitro growth and long-term preservation of potato (foreign No. 2)seedlings under natural or constant temperature conditions. Compared with constant temperature,it was favourable for retard-ing seedlings growth, prolonging in vitro preservation duration and increasing survival rate up to 100%, In addition,it showed the effect of acceleration on in vitro tuberization,with the increasing concentrations of PP_(333),the inhibition of in vitro growth became more intensive and the prolonga-tion of in vitro preservation was evidenced accordingly,the height of plants after 300 days' culture,treated with 10 mg/L PP_(333) was almost the same as that before treatment,treated with PP_(333)seedlings had more leaves, less roots, stout shape and high survival rate,which reduced the trans-ferring times from 4-5per year to 1 per 1-1.5 year. After transferred to culture medinum for re-covery growth,it could be quickly tured back to normal growth after 10 days.

关 键 词：多效唑 马铃薯 试管苗 保存时间 PP333 

分 类 号：S143.8[农业科学—肥料学] S532.043[农业科学—农业基础科学]