甜菜碱醛脱氢酶在菠菜叶细胞中的定位  

Localization of Betaine Aldehyde Dehydrogenase in the Cell of Spinach Leaf

作　　者：骆爱玲[1] 赵原[1] 李浴春 汤岚[1] 梁峥[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《植物生理学报（0257-4829)》1995年第2期117-122,共6页Acta Phytophysiologica Sinica

基　　金：国家自然科学重点基金;北京农业大学植物生理生化开放实验室资助

摘　　要：将菠菜叶片匀浆后．用差速离心和梯度率心分离叶绿体、过氧物酶体、微粒体等细胞器和１０００００×ｇ上清法部分。用酶活测定法测定各部分甜菜碱醛脱氢酶（ＢＡＤＨ）的活性；用免疫扩散法鉴定各组分的ＢＡＤＨ。除叶绿体外，过氧物酶体、微粒体．以及１０００００×ｇ上清液中也存在ＢＡＤＨ。After the cells of spinach leaveswere broken, the chlorplasts, peroxisomes, microsomes, and the 100 000×g supernatant fraction were obtainedby differential centrifugation or gradient density centrifugation. The occurrence of betaine aldehyde dehydrogenase (BADH) in these fractions wastested using two methods: (1 ) assayingthe activity of BADH in each fractionby a spectrophotometric method, and(2 ) immunodetection of BADH ineach fraction with the antiserum against BADH from spinach leaves.BADH was not detected in chloroplastsby the spectrophotometric method(Table 1 ), but found by immunoassaymethods (Figs. 1 and 3). Besided thechloroplasts, BADH was also found inthe other organelles, including perokisomes and microsomes, and in the100 000×g supernatant by the abovetwo methods (Table 1, Figs. 2 and3). These results show that BADH is akind of cytoplasmic enzyme.

关 键 词：甜菜碱醛脱氢酶 定位 细胞器 菠菜 

分 类 号：S636.101[农业科学—蔬菜学]