离体细胞共培养中科间细胞共质体的形成  

FORMATION OF INTERFAMILIAL CELL SYMPLAST DURING CELL CO CULTURE IN VITRO

作　　者：郭骁才[1] 吴伯骥[1] 蒋辉[1] 郑国锠[2] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]兰州大学细胞生物学研究室,兰州730000

出　　处：《Acta Botanica Sinica》1995年第5期339-345,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：中国科学院成都生物研究所所长基金

摘　　要：离体培养下选出的绿色胡萝卜（Ｄａｕｃｕｓ ｃａｒｏｔａ）细胞系和白色普通烟草（Ｎｉｃｏｔｉａｎａｔａｂａｃｕｍ ）细胞系，各自具有独特的细胞结构标志，在愈伤组织、光镜和电镜３ 个水平上均可区分。对两个细胞系进行分散、混合、Ｋ＋ 液低渗处理后在固体培养基上共培养，１０—１５ ｄ 后可观察到两种细胞的镶嵌生长。光镜和电镜下均观察到烟草细胞和胡萝卜细胞之间隔离层的存在与消失。在隔离层消失的区域可见到异种细胞间次生胞间连丝的形成，从而将独立的两个共质体连成一个统一的共质体。对科间细胞共质联系的建立过程进行了讨论，认为细胞接触后首先非特异粘连——以隔离层形成并适度加厚为标志，然后特异的细胞识别在隔离层中启动，从而导致隔离层或消失而重新建立共质联系或加厚、木质化。Two cell lines, white tobacco (Nicotiana tabacum) cell line and green carrot (Daucus carota) cell line, each with very distinct cellular structure markers by which the two cell lines could be identified at levels of callus cells, with light microscopy and electron microscopy, were established and used for interfamilial cell co culture in which callus cells were well mixed and finely dispersed and treated with K + hypotonic solution. Variegated interfamilial chimeral calli were observed after 10 to 15 days of co culture. An isolation layer was formed and became thickened at the interface between the two attached unrelated callus cells and the heteroplastic cell wall complex was gradually established. Then the isolation layer became thinned and disappeared and plasmodesmata were formed secondarily within the thinned region and the interfamilial cell symplast was established. The wall in the region with isolation layer was about twice as thick as the fused cell wall of the symplastic. The developmental process of the interfamilial cell symplastic connection was discussed and it was suggested that (1) the nonspecific formation of isolation layer as initial adhesion between the two attached unrelated cells was the prerequisit for symplastic connection de novo formation, and (2) the specific cell recognition leading to disappearance or thickening to lignification or suberization of isolation layer and formation of plasmodesmata started within the isolation layer.

关 键 词：科间细胞共质体 植物细胞共培养 植物细胞工程 

分 类 号：Q944.6[生物学—植物学] S188[农业科学—农业基础科学]