建立大鼠周边型肺鳞癌模型的方法和体会  

Induction of Peripheral Pulmonary Squamous cell Carcinoma in Rats by Direct Exposure to Chemical Carcinogens

作　　者：王登顺[1] 仇玉福[1] 史景泉[1] 

机构地区：[1]第三军医大学病理解剖学教研室

出　　处：《中国肿瘤临床》1989年第5期313-315,共3页Chinese Journal of Clinical Oncology

摘　　要：本文用甲基胆蒽(MCA)十二乙基亚硝胺(DEN)的碘化油溶液诱发100只Wistar大鼠周边型肺鳞癌。方法上主要是利用气管插管给药后,再灌以0.2ml空气,将致癌剂送至深部气道。系列活杀动物观察结果表明,致癌剂引起的末梢肺组织病变在经历了上皮的立方化、复层化后,发展为浸润性鳞癌。电镜观察结果示这类鳞癌可能起自Ⅱ型肺泡上皮细胞。文中还就本实验中可能遇到的问题及其解决方法进行了讨论。This paper presents the study of MCA+DEN (dissolved in iodized oil) induced peripheral pulmonary squamous cell carcinoma in 100 rats. Carcinogens were propelled into bronchial tree through endotracheal intubation followed by instillation of 0.2 ml of air. Serial observations showed that invasive peripheral squamous cell carcinoma caused by carcinogens were developed subsequent to cuboidization and stratification of the lining epithelial cells in the terminal part of the bronchial trees (including bronchioles, alveolar ducts and alveoli).Electro-microscopic study demonstrated that these peripheral carcinomas might originate from type II alveolar epithelial Cells. The problems encountered in the experiments and their resolution were discussed as well.

关 键 词：肺鳞癌 动物模型 实验肿瘤 

分 类 号：R73－354[医药卫生—肿瘤]