大鼠全脑缺血再灌流过程中几个脑区的自由基体外自旋捕捉ESR测定  

Contents of free radicals with spin trapping in vitro by ESR after reperfusion following ischemia

作　　者：冯加纯 饶明俐 张淑琴 吴江 徐杰[2] 刘桂珍[2] 詹瑞云[2] 

机构地区：[1]长春白求恩医大一院神经科,130021 [2]中科院长春应用化学研究所

出　　处：《中风与神经疾病杂志》1995年第1期2-4,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目

摘　　要：本研究采用自由基自旋捕捉剂α—phenyl—t—butyl nitrone(PBN)进行体外自旋捕捉,用电子自旋共振波谱仪(ESR)测定其加合物含量。动物模型采用wistar大鼠4血管阻断造成全脑缺血10min再灌流模型,分别于缺血10min、再灌5、10、15、30、60、120min,12、24、48、72、120h处死动物,分别取小脑、新皮层、海马、丘脑和纹状体等5个脑区进行体外自旋捕捉。测定结果显示:再灌流后自由基含量明显高于单纯缺血组,其高峰期是再灌流后15min,以后逐渐下降,24h以后已测不出自由基信号。本文还对自由基在脑缺血再灌流过程中产生的机理,及在迟发性神经元坏死发生中的作用机制加以讨论。In this study, contents of free radicals were determined with spin trapping in vitro by electron spin resonance(ESR) in the five brain regions at 10 minutes for global ischemia and 5 minutes to 5 days after reper fusion following ischemia. The spin trap,alpha -phenyl -t-butyl nitrone(PBN) was used, and two line (1x2)ESR spectrum was obtained when a PBN trapped free radical was analyzed. The results showed that contents of free radical increased at ischemia for 10 minutes and 5 minutes to 12 hours after reperfusion. The contents of free radical after reperfusion were higher than that one at ischemia

关 键 词：自旋捕捉 ESR 全脑缺血 再灌流 自由基 脑区 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]