高、低分化的鼻咽癌细胞膜电位比较  

Comparisons of the membrane potentials of the nasopharyngeal carcinoma cells in different degrees of differentiation

作　　者：聂思槐[1] 曾广华[1] 王立伟[1] 陈丽新[1] 钟平[1] 吴枫[1] 胡新荣[1] 唐慰萍[1] 

机构地区：[1]广东医学院神经生理研究室,广东医学院生理学教研室,广东医学院肿瘤研究所

出　　处：《中国病理生理杂志》1995年第3期246-250,共5页Chinese Journal of Pathophysiology

基　　金：广东省高教局重点科研项目

摘　　要：用玻璃微电极细胞内记录法，对体外培养的人胚鼻咽上皮细胞、人低分化鼻咽癌（ＣＮＥ－２Ｚ）和高分化鼻咽癌（ＣＮＥ－１）细胞膜电位（ＭＰ）进行研究，结果表明：（１）与人胚细胞相比，癌细胞ＭＰ负值增大，各细胞ＭＰ差异大，分布弥散，尤以ＣＮＥ－２Ｚ细胞为甚；（２）这３种细胞，细胞外Ｋ￣＋对数浓度与ＭＰ值均呈高度负相关；（３）比较３种细胞ＭＰ值与细胞外液Ｋ￣＋对数浓度的回归系数，ＣＮＥ－２Ｚ的最大，人胚的最小，说明癌细胞尤其是ＣＮＥ－２Ｚ细胞对细胞外液Ｋ￣＋浓度改变更加敏感；（４）培养液Ｎａ￣＋、Ｃａ￣２＋、Ｃｌ￣－浓度增加时，３种细胞ＭＰ改变均无显著性。提示与人胚鼻咽上皮细胞一样，鼻咽癌细胞以细胞内Ｋ￣＋外流为主而形成ＭＰ，但鼻咽癌细胞特别是ＣＮＥ－２Ｚ细胞膜对Ｋ￣＋通透性增大，Ｋ￣＋外流增加而致ＭＰ负值增大。The membrane potentials(MP) of nasopharyngeal epithelial cells of nor-mal human fetus and that of highly and poorly differentiated nasopharyngeal carcinoma(CNE-1 and CNE-2Z)cells in culture were studied by intracellular recording techniques.The results showed:(1)The negative value of the MP of the carcinoma cells,especiallyCNE-2Z cells,increased significantly compared with the normal cells.The difference ofthe MP in different carcinoma cells,especially in CNE-2Z cells was greater than thatof the normal cells and the distribution of MP was more diffuse.(2) In these threekinds of cells lg[K￣+]O was highly negatively correlated to the MP.(3) The regressioncoefficient between MP and lg[K￣+]O in CNE-2Z cells was greatest and that of the nor-mal cells was smallest.These results indicated that the carcinoma cells,especially CNE-2Z cells,were more sensitive to[K￣+]O. Increasing of[Na￣+]O,[Ca￣(2+)]O and [Cl￣-]O didnot change the MP of any cell significantly. These results suggested that although theMP in all these cells are established mainly through the outflow of intracellular K￣+,however the permeability of carcinoma cells,especially of CNE-2Z cells membrane to K￣+increased markedly which leads to the increase of K￣+ outflow and the increased of MPnegative value.

关 键 词：鼻咽肿瘤 细胞 膜电位 离子浓度 

分 类 号：R739.630.2[医药卫生—肿瘤] R730.21[医药卫生—临床医学]