分化诱导剂对人单核样白血病J6－2细胞凝集素结合及［^（125）I］UdR掺入的影响  

Effect of differentiation inducers on the lectin binding capacity of a human monocytoid J6-2 leukemic cells and on the incorporation of [￣125I]UdR into the cell

作　　者：张新波[1] 汤乃梅[1] 夏泉[1] 齐俊明 朱正美[1] 崔肇春[1] 

机构地区：[1]大连医科大学生化教研室,大连海港医院放行科

出　　处：《中国病理生理杂志》1995年第6期582-586,共5页Chinese Journal of Pathophysiology

基　　金：自然科学基金

摘　　要：将Ｊ６－２细胞分别用分化诱导剂ＧＭ＿３（５０μｍｏｌ／Ｌ）、ＤＭＳＯ（１．２％）处理６天、ＴＰＡ（１０ｎｇ／ｍｌ）处理３天，观察它们对１１种凝集素结合反应的影响。结果表明，经ＧＭ＿３处理后，Ｊ６－２细胞对凝集素结合反应增强的只有ＵＥＡ（－至＋），变弱的只有ＢＳＡ（＋＋至－），其余的都无变化。经ＤＭＳＯ处理后，结合反应变强的有ＵＥＡ（－至＋）及ＰＮＡ（－至＋＋），变弱的有ＢＳＡ（＋＋至－）及ＰＳＡ（＋＋至＋）。经ＴＰＡ处理后，结合反应变弱的有ＢＳＡ（＋＋至＋）、ＷＧＡ（＋＋至－）及ＲＣＡ（＋＋至＋），变强的有ＵＥＡ（－至＋）、ＳＢＡ（－至＋）及ＳＪＡ（－至＋＋）。对３种分化诱导剂处理后都无变化的有ＤＢＡ（－）、ＬＣＡ（＋＋）及ＣｏｎＡ（＋＋）。本文还观察到３种分化诱导剂都能抑制［￣１２５Ｉ］ＵｄＲ向Ｊ６－２细胞的掺入。The effect of treating human monocytoid J6-2 leukemic cell s with cell differentiation inducers on the lectin binding capacities and on the incorporation of [￣125I]UdR into J6-2 cells was studied.The lectins used were BSA,ConA,DBA,LCA,PNA,PSA,RCA,SBA,SJA,UEA,and WGA.The results reveal that after 6-day treatment with GM_3(concentration,50μmol/L, the binding capacity( BC )of J6-2 cells for UEA increased(-→+), that for BSA decreased(++→-),while those for others rema- inded unchanged;after 6-day treatment with DMSO (1.2%),the BC for UEA(-→+)and PNA(-→++)increased,those for BSA(++→-)and for PSA (++→+)decreased;after 3-day treatment with TPA(10ng/ml),the BC for UEA (-→+),SBA(-→+)and SJA (-→++)increased, while those for BSA ( ++→+),RCA (++→+), and WGA(++→-)decreased.The BC for DBA (-),LCA ( ++ ),and ConA(++)remained unchanged to treatments with all 3 differentiation inducers.Discussion was directed to the change of carbohydrate structure with respect to change of lectin binding capacity.The results also reveal that after treatment of J6-2 cells with the above mentioned 3 differentiation inducers,the incorporation of[￣125I]UdR into J6-2 cells all decreased.This indicates that the DNA synthesis of J6-2 cells was inhibited by the treatment of the differentiation inducers used in this study.

关 键 词：外源凝集素 神经节甙脂 亚砜类 脱氧尿苷 白血病 

分 类 号：R733.702[医药卫生—肿瘤]