牛磺酸对豚鼠单一心室肌细胞钙离子电流的作用  

EFFECTS OF TAURINE ON CALCIUM CURRENT IN SINGLE VENTRICULAR CELLS OF GUINEA PIGS

作　　者：王丽娟[1] 李金鸣[1] 常天辉[1] 郝丽英[1] 王玉萍[1] 张克义[1] 

机构地区：[1]中国医科大学药理教研室

出　　处：《中国海洋药物》1995年第4期1-5,共5页Chinese Journal of Marine Drugs

摘　　要：应用全细胞膜片钳制技术观察了牛磷酸对豚鼠单一心室肌细胞慢内向钙离子电流(Ica)的作用。实验结果显示:牛磺酸能减少心室肌细胞的Ca^(2+)内流。此作用具有明显的电压依赖性,对其反转电位无明显影响。牛磺酸减少Ica的作用也具有剂量依赖关系,0.1μmol·L^(-1)牛磺酸减小Ica约17％,最大效应浓度接近100μmol·L^(-1)。在停止刺激休息后,首次给予刺激,10μmol·L^(-1)牛磺酸则使Ica减小47％。通过增加刺激频率,牛磺酸能促进Ica的减小。上述结果表明,牛磺酸以电压和使用依赖的方式减少心室肌细胞的钙离子内流。With patch clamp method,the effects of taurine (Tau) on slow inward calcium current (Ica) were investigated in single ventricular cells of guinea pigs. Tau decreased Ica in a dose- and voltage-dependent mode,but had little effect on reversal potential. Tau 0. 1 umol - L-1 decreased Ica by 17% and its maximal effective concentration was near 100 umol -L '. The initial block of Tau had already reached by 47% after a rest out of stimulation. Tau facilitated the reduction of lea through increasing clamp pulse frequency. The obtained results suggested that it decreased Ica in a use-dependent manner.

关 键 词：牛磺酸 钙离子 电流 钙通道阻滞剂 膜片钳 

分 类 号：R972.2[医药卫生—药品]