五种丝虫的氨基酸与化学元素  

STUDIES ON AMINO ACID AND CHEMICAL ELEMENT IN FIVE SPECIES OF FILARIAE

作　　者：陈桂光[1] 李金炎[1] 胡雯[1] 徐礼周[2] 

机构地区：[1]福建医学院 [2]中国农科院家畜寄生虫病研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1995年第1期39-41,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：用高效液相（ＨＰＬＣ）研究人、牛、马、驴、骡与犬体内５种丝虫１８种氨基酸，人、畜丝虫分别含有１６和１７种，缺色氨酸。家畜丝虫氨基酸含量（μｇ／ｍｇ干粉）高于马来丝虫，其酸性和碱性氨基酸（Ｐ＜０．０５）及支链族和芳香族氨基酸（Ｐ＜０．０１）含量高低差异明显并非常显著。以原子吸收分光光度计测定丝虫含Ｚｎ，Ｃｕ，Ｆｅ，Ｍｎ，Ｃｄ５种微量元素中以Ｚｎ为高（μｇ／０．１ｇ干粉），２种常量元素中Ｃａ多于Ｍｇ，这将为研究丝虫（病）的生理代谢与免疫学诊断提供参考资料。Five species of human and domestic animal filariae ( B.m, S.d, S.l, S.e, D.i ) after lyophilization and hydrolysis were resolved using HPLC and type WFX IB AAS to determine amino acid (AA) and microelement (ME) content, respectively. The results showed that there were 16 and 17 AA in B.malayi and the animal filariae, respectively. Among AA, the total amount (μg/mg dry wt.) was markedly higher in animal filariae than that in B.malayi in which the content of acidic and aliphatic AA was significiantly higher than those of basic and aromatic AA, respectively. Among five ME (Zn, Cu, Fe, Mn, Cd) and two microelements (Ca, Mg) found in all these filariae. Zn Content was the predominant ME and Ca was much more than Mg.

关 键 词：丝虫 氨基酸 化学元素 免疫诊断 高效液相色谱 

分 类 号：R383.16[医药卫生—医学寄生虫学]