色素痣和黑素瘤的DNA含量与病理形态学及预后的关系  

Relationship between DNA Content and Pathological Morphology and Prognosis in Pigmented Nevus and Malignant Melanoma

作　　者：丁政云[1] 林元珠[1] 梁祖琪[1] 

机构地区：[1]河北医学院第四医院皮肤科

出　　处：《中国皮肤性病学杂志》1995年第1期7-9,共3页The Chinese Journal of Dermatovenereology

摘　　要：应用流式细胞术（ＦＣＭ）定量研究２５例ＰＮ、２９例ＭＭ及８例皮肤的ＤＮＡ含量和细胞增殖特性。结果表明ＭＭ的ＳＰＦ和ＰＩ显著高于ＰＮ和正常皮肤（Ｐ＜０．０１），二倍体ＭＭ的ＳＰＦ和ＰＩ明显高于ＰＮ（Ｐ＜０．０５），ＤＩ、ＰＩ与ＭＭ组织类型部分有关，ＭＭ的核分裂指数和血管数目与ＤＩ、ＰＩ呈正相关（Ｐ＜０．０５）。提示黑素瘤的ＦＣＭ检测可以鉴别其良恶性，在一定程度上与其组织病理指标相关。Nuclear DNA content and cellular proliferationfeatures of 25 cases of pigmented nevus (PN), 29 malignant melanomas (MM) and 8 normal skin for controlwere quantitatively studied by flow cytometry (FCM).correlations between nuclear DNA content and histological type biological behavior in PN and MM were analyical. All normal skin tissues were DNA-diploid, 21 of25 PN were DNA-diploid, 4 were near diploid andaneluploid (16%). 25 of 29 MM were aneuploid(86%). The aneuploid rate in MM was significantlyhigher than that of PN (P<0. 01 ). S-phase fraction(SPF) (19. 25±3. 41 ) and proliferation index (PI) (33.55±6. 81) in MM were markedly higher that these ofcontrol (8. 33±1. 97; 10. 78±3. 38),and PN (12. 78±3. 72; 21. 63±6. 76), respectively (P < 0. 01; P< 0.01),SPF and PI in MM were notably higher in diploidthan in PN of diploid and near diploid (P<0. 05), Itsuggested that aneuploid may be regarded as a markerof MM:SPF and PI may be veluable in reflecting themalignant degree and growth behavior of tumors and indistinguishing benign from malignant tumor. From thisstudy we suggest that FCM-DNA may be a valuable indicator in differential diagnosis and prognosis of benignand malignant melanocytic tumors.

关 键 词：病理形态学 预后 色素痣 黑色素瘤 预后 

分 类 号：R739.5[医药卫生—肿瘤]