用PCR扩增技术检测卡氏肺孢子虫DNA  

Detection of Pneumocystis Carinii DNA of Experimentally Infected Rats by PCR

作　　者：余海昕 邝丽贤 刘启文[1] 

机构地区：[1]中山医科大学寄生虫学教研室

出　　处：《中国人兽共患病杂志》1995年第4期8-10,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金

摘　　要：本文报道了用ＰＣＲ扩增技术检测卡氏肺孢子虫感染大鼠的肺组织、支气管灌洗液和外周血病原体ＤＮＡ，并和肺印片结果进行比较。共检测了实验感染鼠３４只及正常大鼠６只，肺印片阳性者１８例，ＰＣＲ检测肺组织样品出现特异的扩增条带者１９例，检测支气管灌洗液样品出现特异的扩增条带者１６例，外周血样品全部阴性。根据实验结果，认为此法用于检测卡氏肺孢子虫病原体ＤＮＡ有很好的应用前景。Polymerase chain reaction was employed to detect the Pneumocystis carinii DNA in the lung,broncho lavage and peripheral serum from 36 experimentally infected rats and 6 normal rats.Among the 36 rats treated with Dexamethasoni,18 showed the presence of P.carinii cysts and trophozoites of which the PCR band from the lung and broncho lavage were shown in19 and 16 rats respectively.No PCR band showed from serum of the infected rats and samples from normal rats.It suggested that PCR should be a promissing technique for diagnosis of PCP.

关 键 词：多聚酶链反应 卡氏肺孢子虫 孢子虫 DNA 

分 类 号：R382.3[医药卫生—医学寄生虫学]