体外培养细胞中特异反义RNA表达对猪瘟病毒增殖的抑制作用  

Inhibition of Hog Cholera Virus by the Expression of Spe-cific Antisense RNA in Cells

作　　者：章金钢[1] 涂长春[1] 金扩世[1] 李红卫[1] 胡敬东 扈荣良[1] 戴秉丽 殷震[1] 

机构地区：[1]长春农牧大学军事兽医研究所

出　　处：《中国兽医学报》1995年第1期4-9,共6页Chinese Journal of Veterinary Science

基　　金：国家"863"计划和国家自然科学基金

摘　　要：应用免疫荧光抗体技术检测了整合有猪瘟病毒反义基因的ＰＫ－１５细胞克隆对猪瘟病毒的抑制效应。结果表明，５个不同的反义基因片段对猪瘟病毒的抑制效率存在很大的差异，其中Ａ片段的抑制效率最高（９４％～９８％），Ｂ片段次之（５８％～７６％），Ｃ片段再次（６４％以下），Ｄ片段和Ｅ片段未见明显的抑制效应。抑制效率的差异可能与反义基因片段的位置、长短以及反义ＲＮＡ表达质粒载体有关。The specific inhibition of hog chloera virus(HCV)replication by anti-sense RNA expressed in cells transfected with HCV antisense gene constructs wasdemonstrated by immunofluorescent assay(IFA)at the presence of Zn2+(50 or 65μmol/L)as inducer of metallothionein-I promoter.The inhibition rate in mMT/HCA,mMT/HCB and mMT/HCC(using mouse MT-I)transfected cell clones were 94%- 98%,58%-76%and-64%respectively.No apparent inhibition was observed in pMT/HCD and pMT/HCE (using ovine MT-I)transfected clones.The reasons for the differ-ences in inhibition effects may be resulted from the sizes and positions of the HCV anti-sense gene fragments or the as RNA expressing vectors.

关 键 词：猪瘟病毒 反义基因 病毒抑制 

分 类 号：Q78[生物学—分子生物学] S852.651[农业科学—基础兽医学]