我国肠道腺病毒的分离与鉴定  

Isolation and Identification of Enteric Adenovirus in China

作　　者：程绪杰[1] 王树蕙[1] 张云[1] 赵锦铭 郭彩云[1] 

机构地区：[1]中国医学科学院,中国协和医科大学,北京市首都儿科研究所

出　　处：《中国医学科学院学报》1995年第1期16-19,共4页Acta Academiae Medicinae Sinicae

摘　　要：在国内首次从腹泻患儿粪便标本中分离得到一株病毒８６－１２３，其感染Ｇｒａｈｍ２９３细胞后，出现明显的细胞病变；进一步用电镜和ＧａｍｂｒｉｄｇｅＢｉｏｔｅｃｈＡｄｅｎｏｃｌｏｎｅ－ｔｙｐｅ４０／４１特异性诊断试剂盒检测，结果证明８６－１２３为肠道腺病毒，该工作为肠道腺病毒感染的预防和诊断及其病原学研究打下了坚实的基础。Enteric adenovirus type 40/41 is cosidered to be the second major cause of gastoenteritis in young children.In this study fecal specimens 86-123 from diarrhea patients were isolated and examined in Grahm 293 cells. This induced cytopathic effect(CPE)at this cells.Viral particles were also found in fecal specimens and by electron microscopy. Examination of the isolate with Cambridge Biotech Adenoclone-Type 40/41 test kit indicated that it contained EAd. On the basis of the above studies, one strain of EAdwas the first isolated virus in China.

关 键 词：肠道腺病毒 诊断试剂盒 分离 鉴定 

分 类 号：R373.2[医药卫生—病原生物学]