牦牛牛病毒性腹泻病毒P20和P14基因的克隆及序列分析  

Cloning and sequence analysis of the p20 and p14 gene of bovine viral diarrhea virus Yak strain

作　　者：冯英阳[1] 刘亚刚[1] 于吉锋[1] 常文棣[1] 胡炳峰[1] 王文伯[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《四川大学学报（自然科学版）》2009年第6期1805-1810,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省科技厅攻关项目(04ZY029-006-04);西南民族大学研究生创新型科研项目

摘　　要：参考GenBank中多个BVDV毒株基因组序列,利用生物软件primer5.0设计两对引物,扩增出BVDV牦牛株P20基因和P14基因.结果表明,克隆得到的P20基因序列为504bp,与GenBank上发表的BVDV P20大小一致,P14基因序列为312 bp,与GenBank上发表的BVDV毒株比较有6个插入碱基.核苷酸序列的同源性及系统发生分析的结果表明,牦牛(Yak)株属于BVDV-1.According to the sequence of BVDV strains published by Genbank,two set of primers were designed by primer premier 5.0 and used to amplify by PCR .The p20 and p14 gene of BVDV in Yak were firstly cloned.Two specific 504bp and 312bp DNA product were amplified .The result confirms that the length of p20 gene is accordant with other BVDV strains published by Genbank,and the Yak strain possess six base insertion in the p14 gene region.The Yak strain of BVDV belongs to genotype Ⅰ by nucleotide homololgy analyses and the phylogenetic analyses.

关 键 词：牦牛 牛病毒性腹泻病毒 P14基因 克隆 序列分析 BVDV GENBANK P20基因 系统发生分析 sequence of 基因组序列 核苷酸序列 生物软件 结果 毒株 GENOTYPE product 同源性 Two 引物 

分 类 号：N55[自然科学总论]