枯草芽孢杆菌LL18-4产类细菌素的分离纯化及特性研究  

The purification and partial characterization of a bacteriocin-like antifungal substance from Bacillus subtilis

作　　者：孙苗苗[1] 赵昆[1] 武志芳[1] 唐德芳[1] 胡承[1] Miao-Miao Zhi-Fang De-Fang 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2009年第6期1862-1866,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：省科技厅科技攻关项目(04NG003-007-1)

摘　　要：枯草芽孢杆菌(Bacillus subtilis)LL18-4菌株分泌物中的类细菌素通过硫酸铵盐析法粗提后,经过Sephadex G-75、DEAE-Sephadex A 50和Sephacryl S-200HR分步柱层析,SDS-PAGE电泳检测到单一蛋白质条带,分子量约为60 kD.该类细菌素在酸性条件下及80%饱和度的硫酸铵溶液中具有较高的稳定性,可4℃放置3个月仍保持80%以上活性;对有机溶剂有一定的耐受性;Mg^(2+)能提高其抑菌活性.指数增长前期添加柑桔绿霉(Peniicilbumdiigitatum)病原真菌的培养液可以提高该类细菌素的分泌量.A bacteriocin-like substance(BLS) produced by Bacillus subtilis LL18-4 was purified via ammonnium sulphate precipitation,Sephadex G-75,DEAE-Sephadex A 50 and Sepharyl S-200 HR chromatography, and in the final purification step,one active fraction was obtained and the molecular weight of the purified BLS determined by SDS-PAGE was about 60 kD.The BLS was able to resist the denaturation action of several organic solvents and the antifungal activity was increased when incubated with Mg^(2+).The fermentation ability of LL18-4 was improved when the fermentation of Peniicilbum diigitaturn was added in the early exponential growth phase.

关 键 词：枯草芽孢杆菌 类细菌素 分离纯化 特性研究 Bacillus SUBTILIS SEPHADEX antifungal activity SDS-PAGE电泳 organic solvents molecular weight active fraction 硫酸铵溶液 蛋白质条带 BLS 指数增长 有机溶剂 抑菌活性 酸性条件 过硫酸铵 病原真菌 

分 类 号：N55[自然科学总论]