银屑病患者外周血T细胞CD69和CD134表达的研究  

Expression of CD69 and CD134 on psoriatic peripheral blood T lymphocytes

作　　者：刘毅[1] 蔡小嫦[2] 曾耀英[3] 

机构地区：[1]北京协和医学院皮肤病研究所 中国医学科学院,210042 [2]暨南大学附属第一医院皮肤科 [3]暨南大学生命科学技术学院组织移植与免疫实验中心

出　　处：《中国实用医刊》2010年第11期8-11,共4页Chinese Journal of Practical Medicine

摘　　要：目的 了解银屑病患者外周血T细胞CD69和CD134的表达情况及多克隆刺激剂对其表达的影响.方法 取银屑病患者与健康志愿者外周血全血培养,佛波醇脂(PMA)联合Ionomycin刺激使T细胞体外活化;在刺激第0、6、12、24 h,用三色免疫荧光标记流式细胞术检测T细胞CD69、CD134的表达.结果 银屑病患者组和健康对照组各11例纳入研究,结果显示:①银屑病患者组新鲜血标本T细胞CD134、CD69表达率均明显高于健康对照组(P<0.01); ②PMA联合Ionomycin刺激第6、12、24 h,银屑病患者组与健康对照组外周血T细胞CD69表达率仅在第24 h差异具有统计学意义(P<0.05); ③在刺激第6、12、24 h,银屑病患者组外周血T细胞CD134表达率均较健康对照组显著升高(P<0.001).结论 银屑病患者外周血T细胞存在异常活化.在体外活化的外周血T细胞上,银屑病患者组CD134的表达显著高于健康对照组.Objective To investigate the in vivo expression of CD134 by the peripheral blood T cells from psoriasis patients, the regulation of their expression in response to polyclone stimulator. Methods The whole blood culture procedure was applied to mimic the natural conditions of T cell activation in vivo. T cells were activated by polyclone stimulator PMA plus Ionomycine. CD69 and CD134 expressed in vitro on activated T lymphocytes from psoriasis patients and healthy controls were detected with three-color flow cytometry. Results The results showed that the expression rates of CD134 and CD69 on the peripheral T cells from psoriasis patients were much higher than those of healthy controls (P<0.05). In response to the stimulation of PMA plus Ionomycin, there was only significant difference on the expression rates of CD69 on the peripheral blood T cells between these two groups at 24 h post activation. Whereas, the expression rates of CD134 on psoriatic peripheral blood T cells were significantly increased than those of the healthy controls at all time points (P<0.001). Conclusions The results verified the aberrant activation of the peripheral blood T lymphocytes in psoriasis. Moreover, the expression of CD134 on psoriatic peripheral T cells in response to polyclone stimulators PMA plus Ionomycin is in a stronger manner than those of healthy controls.

关 键 词：银屑病患者 外周血 流式细胞术检测 CD69 表达率 T lymphocytes peripheral BLOOD T cells controls 对照组 健康志愿者 results 刺激 体外活化 PMA 免疫荧光标记 plus whole BLOOD significant 统计学意义 

分 类 号：R758[医药卫生—皮肤病学与性病学]