Hemin对Bel/Fu肝癌耐药细胞株HO-1、GST-π及化疗敏感性的影响  

HEMIN′S EFFECT ON HO-1/GST-π EXPRESSION AND CHEMOSENSITIVITY OF DRUG-RESISTANT HEPATIC CARCINOMA CELL LINE

作　　者：张隽开[1] 王忠裕[1] 罗海峰[1] 程雷[1] 牟国煜[1] 

机构地区：[1]大连医科大学附属一院普外科,大连116011

出　　处：《肝胆外科杂志》2011年第1期64-66,共3页Journal of Hepatobiliary Surgery

摘　　要：目的评估氯化高铁血红素(Hemin)对肝癌耐药细胞株血红素加氧酶-1(Heme Oxygenase,HO-1)、谷胱甘肽-S-转移酶GST-π(Glutathione S-transferase,GST-π)的影响,探讨Hemin作用对GST-π调节化疗敏感性的可能机制。方法不同浓度的Hemin作用于Bel/Fu肝癌化疗耐药细胞株,分别应用MTT、RT-PCR检测细胞株对化疗药物敏感性、HO-1与GST-π核酸水平表达量。结果 Hemin作用于Bel/Fu细胞诱导24小时后,化疗药物半数抑制浓度明显增加,HO-l mRNA表达明显增加(F=71.513,其中2umol/L组P=0.029,余均<0.01),GST-π mRNA的表达量也显著增加(各P值均<0.01),均呈剂量依赖趋势。结论氯化高铁血红素Hemin在诱导HO-1表达的同时,通过影响GST-π的表达,使肝癌耐药细胞株的化疗敏感性降低;HO-1可作为逆转肿瘤多药耐药的潜在作用靶点。Objective To evalue Hemin,s effect on the expression of Heme Oxygenase(HO-1) and Glutathione S-transferase(GST-π)and the chemosensitivity of drug-resistant hepatic carcinoma Bel/Fu cell line,explore the relevant regulating mechanism. Methods After induced with different gradient concentrations of Hemin for 24h,heme oxygenase-1 and Glutathione S-transferase mRNA level were determined by RT-PCR technique; The drug sensitivity test of Bel/Fu was carried out with MTT assay. Results After Bel/Fu cells being deal...

关 键 词：肝肿瘤细胞 谷胱甘肽-S-转移酶 血红素加氧酶-1 化疗耐药性 

分 类 号：R735.7[医药卫生—肿瘤]