山羊转基因克隆胚在体内和体外发育的研究  

Study on Development of Goat Transgenic-cloned Embryos Cultivated in Vivo and in Vitro

作　　者：张果平[1] 黄庆华[1] 王可[1] 王金文[1] 王德芹[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室,山东济南250100

出　　处：《江西农业学报》2010年第8期125-128,共4页Acta Agriculturae Jiangxi

基　　金：山东省农业科学院青年科研基金(2007Q28);国家公益行业(农业)专项经费(3-45)资助

摘　　要：探讨了体内/外成熟卵母细胞、体内/外培养系统、输卵管液和输卵管上皮细胞共培养系统对转基因克隆胚发育的影响。结果表明:体内/外成熟卵母细胞对转基因克隆胚各阶段发育率无显著影响。体内/外培养系统中,转基因克隆胚2～3-细胞、4-细胞、8-细胞发育率差异不显著;16-细胞和桑/囊胚发育率差异显著。对照组和20%输卵管液中,转基因克隆胚2～3-细胞、4-细胞、8-细胞发育率均显著高于50%输卵管液;在20%输卵管液中16-细胞的发育率显著高于对照组和50%输卵管液。转基因克隆胚在输卵管上皮细胞共培养系统和SOF培养系统中培养,2～3-细胞和4-细胞发育率无显著差异;8-细胞、16-细胞和桑/囊胚的发育率差异显著。The effects of IVO/IVM oocytes,culture system in vivo/vitro,oviduct fluid,and co-culture with oviduct epithelia on the development of goat transgenic-cloned embryos were explored.The results demonstrated that there was no significant difference in the effect of matured oocytes in vivo/vitro on the development rate of transgenic-cloned embryos at all stages(P>0.05).There was no significant difference in development rate of 2～3-cell,4-cell,and 8-cell of transgenic-cloned embryos cultivated in vivo or in vitro(P>0.05).The 16-cell development and morula/blast rate of transgenic-cloned embryos cultivated in vivo were very higher than those of embryos cultivated in vitro(P<0.05).The development rate of 2～3-cell,4-cell,and 8-cell of transgenic-cloned embryos cultivated in control and 20% oviduct fluid was higher than that cultivated in 50% oviduct fluid(P<0.05).The development rate of 16-cell and morula/blast rate of transgenic-cloned embryos cultivated in 20% oviduct were higher than those cultivated in control and 50% oviduct fluid(P<0.05).There was no significant difference in development rate of 2～3-cell and 4-cell of transgenic-cloned embryos cultivated in SOF or in co-culture with oviduct epithelia(P>0.05).The development rate of 8-cell,16-cell and morula/ blast rate of transgenic-cloned embryos cultivated in co-culture with oviduct epithelia were higher than those cultivated in SOF(P<0.05).

关 键 词：山羊 基因克隆 体内 体外发育 in VITRO Development 输卵管上皮细胞 囊胚发育率 输卵管液 共培养系统 成熟卵母细胞 差异显著 对照组 显著差异 结果 SOF 

分 类 号：S[农业科学]