银掺杂聚L-酪氨酸修饰电极同时测定多巴胺、肾上腺素和抗坏血酸  

Simultaneous Determination of Dopamine,Epinephrine and Ascorbic Acid on Silver Doped Poly(L-Tyrosine) Modified Electrode

作　　者：孙登明[1] 田相星[1] 马伟[1] 

机构地区：[1]淮北师范大学化学与材料科学学院,淮北235000

出　　处：《分析化学》2010年第12期1742-1746,共5页Chinese Journal of Analytical Chemistry

基　　金：安徽省高校省级自然科学研究重点项目基金(No.KJ2008A122);安徽省含能材料重点实验室科学研究基金(No.KLEM2009008)资助项目

摘　　要：用循环伏安法制备银掺杂聚L-酪氨酸修饰玻碳电极,研究了多巴胺、肾上腺素和抗坏血酸在其电极上的电化学行为,建立了同时测定多巴胺、肾上腺素和抗坏血酸的新方法。当3种组分共存时,在磷酸盐缓冲溶液(pH6.0)中,扫描速率为140mV/s,多巴胺和肾上腺素在修饰电极上分别产生还原峰,峰电位分别为0.198和-0.205V,多巴胺和肾上腺素氧化峰重叠,峰电位为0.313V(vs.Ag/AgCl);抗坏血酸产生一个氧化峰,峰电位0.108V(vs.Ag/AgCl)。多巴胺和肾上腺素的ΔEpc=0.403V,抗坏血酸的氧化峰与多巴胺和肾上腺素的ΔEpa=0.205V,用还原峰和氧化峰可同时测定多巴胺、肾上腺素和抗坏血酸,3种组分同时测定的线性范围分别为5.0×10-6～1.0×10-4mol/L,8.0×10-6～1.0×10-4mol/L和3.0×10-5～1.0×10-3mol/L;检出限分别为5.0×10-7,8.0×10-7和5.0×10-6mol/L。本方法用于人尿液中多巴胺、肾上腺素和抗坏血酸的同时测定,结果满意。Silver doped poly(L-tyrosine) modified electrode was prepared by cyclic voltammetric method.Voltammetric behavior and determination method for dopamine(DA),epinephrine(EP),ascorbic acid(AA) were studied on the modified electrode.In pH 6.0 phosphate buffer solution,the modified electrode gave two cathodic peaks at 0.198 V and-0.205 V(vs.Ag/AgCl) for DA and EP,two oxidation peaks overlaps at 0.313 V(vs.Ag/AgCl) for DA and EP,one oxidation peak at 0.108 V(vs.Ag/AgCl) for AA.Two separated cathodic peaks were divided 0.403 mV for DA and EP,the oxidation of AA and the overlapped xidation peaks of EP and DA was divided 0.205 mV.The linear response was obtained in the range of 5.0 × 10-6-1.0 × 10-4 mol/L for DA,8.0 × 10-6-1.0 × 10-4 mol/L for EP and 3.0 × 10-5-1.0 × 10-3 mol/L for AA.The detection limits were 5.0 × 10-7 mol/L for DA,8.0 × 10-7 mol/L for EP and 5.0 × 10-6 mol/L for AA,respectively.The method was successfully applied to the simultaneous determination of DA,EP and AA in human urine samples with satisfactory results.

关 键 词：银掺杂 酪氨酸 修饰电极 同时测定 多巴胺 肾上腺素 抗坏血酸 Determination of Poly 氧化峰 峰电位 磷酸盐缓冲溶液 修饰玻碳电极 还原峰 电化学行为 循环伏安 线性范围 扫描速率 新方法 人尿液 

分 类 号：R927[医药卫生—药学]